时门控荧光显微镜结合响应性非金属探针用于活细胞中蛋白激酶活性的定位。
Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells.
机构信息
Institute of Chemistry, University of Tartu, 14A Ravila St., 50411 Tartu, Estonia.
出版信息
Chem Commun (Camb). 2012 Sep 4;48(68):8595-7. doi: 10.1039/c2cc33565d. Epub 2012 Jul 20.
Abstract
A photoluminescence probe ARC-1185, possessing both high affinity towards basophilic protein kinases (PKs) and microsecond-scale luminescence lifetime when associated with a kinase, was used for the mapping of ARC-1185-PK complexes in living cells with time-gated luminescence microscopy.
摘要
一种具有高亲和力的荧光探针 ARC-1185，在与激酶结合时具有微秒级的荧光寿命，被用于通过门控时间荧光显微镜对活细胞中的 ARC-1185-PK 复合物进行定位。
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