Ewald S, Heuvelman C J, Notermans S
Department of Food Hygiene, Norwegian College of Veterinary Medicine, Oslo.
Int J Food Microbiol. 1990 Dec;11(3-4):251-7. doi: 10.1016/0168-1605(90)90018-z.
DNA-DNA colony hybridization was employed to evaluate the results obtained by different immunological methods for detection of staphylococcal enterotoxin. Staphylococcus aureus strains tested for staphylococcal enterotoxin production by immuno-assays and micrococci not previously tested for staphylococcal enterotoxin production were examined for presence of the genes encoding for staphylococcal enterotoxin A, B, C and E by using three corresponding DNA probes. The staphylococcal enterotoxin A probe also detected staphylococcal enterotoxin E gene because of 100% homology. The optimal sensitivity plate method showed the best accordance between the immuno-assay and the hybridization reactions. The enzyme-linked immunosorbent assay detected 12.5 to 17% staphylococcal enterotoxin producers without hybridization reactions. The microslide gel double diffusion test and the reversed passive latex agglutination test showed rather poor accordance with the hybridization reactions. All 17 strains of different micrococci investigated were negative in hybridization with all three DNA probes.
采用DNA - DNA菌落杂交法评估不同免疫方法检测葡萄球菌肠毒素所获得的结果。通过免疫测定法检测产葡萄球菌肠毒素的金黄色葡萄球菌菌株，以及之前未检测过产葡萄球菌肠毒素的微球菌，使用三种相应的DNA探针检测编码葡萄球菌肠毒素A、B、C和E的基因的存在情况。由于100%的同源性，葡萄球菌肠毒素A探针也检测到了葡萄球菌肠毒素E基因。最佳灵敏度平板法显示免疫测定法与杂交反应之间的一致性最好。酶联免疫吸附测定法检测到12.5%至17%的产葡萄球菌肠毒素菌株无杂交反应。微量玻片凝胶双向扩散试验和反向被动乳胶凝集试验与杂交反应的一致性较差。所研究的17株不同微球菌与所有三种DNA探针杂交均为阴性。
