金黄色葡萄球菌菌株单反应多重PCR毒素分型检测方法的开发
Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains.
作者信息
Sharma N K, Rees C E, Dodd C E
机构信息
Division of Food Sciences, School of Biological Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom.
出版信息
Appl Environ Microbiol. 2000 Apr;66(4):1347-53. doi: 10.1128/AEM.66.4.1347-1353.2000.
Abstract
We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the assay can detect enterotoxin genes A to E from a pure culture within 3 to 4 h. The test was used to characterize a diverse set of environmental S. aureus isolates, and a 99% correlation with toxin typing using standard immunological tests was found. The design of the assay allows it to be extended to include both newly characterized and as-yet-unknown toxin genes.
摘要
我们在此描述一种用于检测金黄色葡萄球菌肠毒素基因的单反应多重聚合酶链反应（PCR）检测方法的开发，该方法利用通用毒素基因引物与毒素特异性引物相结合来扩增特征性毒素基因产物。结合一种新的DNA纯化方法，该检测方法能够在3至4小时内从纯培养物中检测出A至E型肠毒素基因。该检测方法用于对多种环境来源的金黄色葡萄球菌分离株进行特征分析，发现与使用标准免疫学检测进行的毒素分型有99%的相关性。该检测方法的设计使其能够扩展到包括新鉴定的和尚未知晓的毒素基因。
相似文献
1
Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains.
Appl Environ Microbiol. 2000 Apr;66(4):1347-53. doi: 10.1128/AEM.66.4.1347-1353.2000.
2
Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates.
FEMS Microbiol Lett. 2005 May 15;246(2):191-8. doi: 10.1016/j.femsle.2005.04.007.
3
Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene.
J Clin Microbiol. 1998 Sep;36(9):2548-53. doi: 10.1128/JCM.36.9.2548-2553.1998.
4
Development of a multiplex-PCR for direct detection of the genes for enterotoxin B and C, and toxic shock syndrome toxin-1 in Staphylococcus aureus isolates.
J Med Microbiol. 1998 Apr;47(4):335-40. doi: 10.1099/00222615-47-4-335.
5
Detection of Staphylococcus aureus enterotoxins A to D by real-time fluorescence PCR assay.
J Clin Microbiol. 2003 Oct;41(10):4683-7. doi: 10.1128/JCM.41.10.4683-4687.2003.
6
Application of extended single-reaction multiplex polymerase chain reaction for toxin typing of Staphylococcus aureus isolates in South Korea.
Int J Food Microbiol. 2004 Dec 15;97(2):137-45. doi: 10.1016/j.ijfoodmicro.2004.04.014.
7
Investigation of toxin genes by polymerase chain reaction in Staphylococcus aureus strains isolated from bovine mastitis in Turkey.
Foodborne Pathog Dis. 2009 Oct;6(8):1029-35. doi: 10.1089/fpd.2009.0304.
8
Detection and genotyping by real-time PCR of the staphylococcal enterotoxin genes sea to sej.
Mol Cell Probes. 2003 Aug;17(4):139-47. doi: 10.1016/s0890-8508(03)00045-8.
9
[Enterotoxin genes occurance among S. aureus strains isolated from inpatients and carriers].
Med Dosw Mikrobiol. 2006;58(4):275-81.
10
Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York.
Appl Environ Microbiol. 2009 Nov;75(21):6839-49. doi: 10.1128/AEM.00272-09. Epub 2009 Sep 11.
引用本文的文献
1
Effect of feed supplementation with humic substances on phenotypic resistance profiles of Escherichia coli isolates from the ceca of broiler chickens.
Poult Sci. 2025 Apr 16;104(7):105175. doi: 10.1016/j.psj.2025.105175.
2
Phylogenetic analysis of enterotoxin A gene in Iraqi breed cows with bovine mastitis: Implications for disease management.
Open Vet J. 2024 Jul;14(7):1644-1657. doi: 10.5455/OVJ.2024.v14.i7.14. Epub 2024 Jul 31.
3
Pathogenic potential of meat-borne coagulase negative staphylococci strains from slaughterhouse to fork.
Int Microbiol. 2024 Dec;27(6):1781-1793. doi: 10.1007/s10123-024-00500-2. Epub 2024 Mar 23.
4
spa Types and Staphylococcal Enterotoxin Production of Staphylococcus aureus Isolated from Wild Boar.
Microb Ecol. 2023 Oct;86(3):2184-2191. doi: 10.1007/s00248-023-02236-4. Epub 2023 May 9.
5
Virulence Potential and Antibiotic Susceptibility of Strains Isolated from Food Handlers.
Microorganisms. 2022 Oct 30;10(11):2155. doi: 10.3390/microorganisms10112155.
6
Detection of Gluten in Gluten-Free Foods of Plant Origin.
Foods. 2022 Jul 7;11(14):2011. doi: 10.3390/foods11142011.
7
Detection of Resistant and Enterotoxigenic Strains of Isolated from Food of Animal Origin.
Foods. 2022 May 20;11(10):1496. doi: 10.3390/foods11101496.
8
Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks.
Front Microbiol. 2021 Nov 2;12:750278. doi: 10.3389/fmicb.2021.750278. eCollection 2021.
9
Occurrence and Characteristics of in a Hungarian Dairy Farm during a Control Program.
Pathogens. 2021 Jan 21;10(2):104. doi: 10.3390/pathogens10020104.
10
Antibiotic Resistance and Virulence Gene Characteristics of Methicillin-Resistant (MRSA) Isolated from Healthy Edible Marine Fish.
Int J Microbiol. 2020 Jun 4;2020:9803903. doi: 10.1155/2020/9803903. eCollection 2020.
本文引用的文献
1
Plasmid Profiles and Pulsed-Field Gel Electrophoresis for Type A Enterotoxigenic Staphylococcus aureus Isolated from Foods.
J Food Prot. 1995 Feb;58(2):147-153. doi: 10.4315/0362-028X-58.2.147.
2
Enterotoxigenicity of Staphylococcus aureus Strains Isolated from Chickens.
J Food Prot. 1980 Sep;43(9):683-685. doi: 10.4315/0362-028X-43.9.683.
3
Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates.
J Clin Microbiol. 1999 Oct;37(10):3411-4. doi: 10.1128/JCM.37.10.3411-3414.1999.
4
The enterotoxin D plasmid of Staphylococcus aureus encodes a second enterotoxin determinant (sej).
FEMS Microbiol Lett. 1998 Nov 15;168(2):227-33. doi: 10.1111/j.1574-6968.1998.tb13278.x.
5
Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene.
J Clin Microbiol. 1998 Sep;36(9):2548-53. doi: 10.1128/JCM.36.9.2548-2553.1998.
6
Identification and characterization of staphylococcal enterotoxin types G and I from Staphylococcus aureus.
Infect Immun. 1998 Jul;66(7):3337-48. doi: 10.1128/IAI.66.7.3337-3348.1998.
7
Development of a multiplex-PCR for direct detection of the genes for enterotoxin B and C, and toxic shock syndrome toxin-1 in Staphylococcus aureus isolates.
J Med Microbiol. 1998 Apr;47(4):335-40. doi: 10.1099/00222615-47-4-335.
8
Production of enterotoxins and toxic shock syndrome toxin by bovine mammary isolates of Staphylococcus aureus.
J Clin Microbiol. 1993 Mar;31(3):706-7. doi: 10.1128/jcm.31.3.706-707.1993.
9
Characterization and biological properties of a new staphylococcal exotoxin.
J Exp Med. 1994 Nov 1;180(5):1675-83. doi: 10.1084/jem.180.5.1675.
10
Patterns of Staphylococcus aureus colonization, toxin production, immunity and illness in burned children.
Burns. 1994 Dec;20(6):514-21. doi: 10.1016/0305-4179(94)90011-6.
Zotero 插件