胱氨酸负荷及胱氨酸二甲酯对肾刷状缘膜转运的影响。

Effect of cystine loading and cystine dimethylester on renal brushborder membrane transport.

作者信息

Foreman J W, Benson L

机构信息

Department of Pediatrics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.

出版信息

Biosci Rep. 1990 Oct;10(5):455-9. doi: 10.1007/BF01152292.

DOI:10.1007/BF01152292

PMID:2282372

Abstract

The effect of loading renal tubule cells with cystine was studied by incubating them with cystine dimethylester. Proline uptake into brushborder membrane vesicles isolated from the cystine loaded cells was not different from that observed into brushborder vesicles isolated from tubules incubated in buffer alone. Incubating brushborder membranes with 2 mM cystine dimethylester for 10 minutes reduced the uptake of proline by 27% after 15 seconds of incubation and by 21% after 60 seconds of incubation. There was no effect after 20 minutes of incubation. Pre-incubating brushborder membrane vesicles with cystine dimethylester had no statistically significant effect on the affinity of proline for the carrier, but did reduce the maximal rate of proline uptake by 49%.

摘要

通过用胱氨酸二甲酯孵育肾小管细胞来研究胱氨酸加载对肾小管细胞的影响。从加载胱氨酸的细胞中分离出的刷状缘膜囊泡对脯氨酸的摄取与仅在缓冲液中孵育的肾小管分离出的刷状缘囊泡中的摄取情况没有差异。用2 mM胱氨酸二甲酯孵育刷状缘膜10分钟后，孵育15秒时脯氨酸摄取减少27%，孵育60秒时减少21%。孵育20分钟后没有影响。用胱氨酸二甲酯预孵育刷状缘膜囊泡对脯氨酸与载体的亲和力没有统计学上的显著影响，但确实使脯氨酸摄取的最大速率降低了49%。