Hasan M M, Hossain M A, Paul S K, Mahmud C, Khan E R, Rahman M M, Rukunuzzaman M, Hasan M S, Kubayashi N
Sherpur Sadar General Hospital, Sherpur, Bangladesh.
Mymensingh Med J. 2012 Jul;21(3):399-403.
Tuberculosis (TB) is a major public health problem in most developing countries. The present study was carried out among 100 clinically suspected pulmonary TB patients. One hundred sputum specimens were collected one from each of the suspects attending DOT'S corner of Mymensingh Medical College Hospital, Mymensingh, Bangladesh. The aim of the study was to evaluate the sensitivity and specificity of a polymerase chain reaction (PCR) based method detecting IS6110 sequence present in all Mycobacterium tuberculosis strains using sputum samples in comparison to culture on Lowenstein-Jensen mediums. The PCR was done using primers mtb1 & mtb2 which commonly target an insertion sequence of the organism (IS6110). Out of 100 samples, 18 (18%) showed PCR positive, whereas culture in Lowenstein-Jensen media were positive in 19(19%). In PCR 1 was false negative but none was false positive. In present study, sensitivity and specificity of PCR found 94.74% and 100% respectively. Analyzing the findings of the present study, it can be concluded that the PCR technique is a rapid and alternative method of culture on Lowenstein-Jensen medium for the diagnosis of pulmonary tuberculosis. In the present study, only presence or absence of M. tuberculosis was determined.
结核病是大多数发展中国家的一个主要公共卫生问题。本研究在100例临床疑似肺结核患者中开展。从孟加拉国迈门辛医学院医院迈门辛分院直接观察治疗点的每位疑似患者处收集了100份痰标本。本研究的目的是评估一种基于聚合酶链反应（PCR）的方法的敏感性和特异性，该方法使用痰标本检测所有结核分枝杆菌菌株中存在的IS6110序列，并与在罗-琴培养基上培养的结果进行比较。PCR使用引物mtb1和mtb2进行，这两种引物通常靶向该生物体的一个插入序列（IS6110）。在100份样本中，18份（18%）显示PCR阳性，而在罗-琴培养基上培养呈阳性的有19份（19%）。在PCR检测中有1例假阴性，但无假阳性。在本研究中，PCR的敏感性和特异性分别为94.74%和100%。分析本研究的结果可以得出结论，PCR技术是一种用于诊断肺结核的快速且可替代在罗-琴培养基上培养的方法。在本研究中，仅确定了结核分枝杆菌的有无。
