Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
Biotechnol Lett. 2012 Nov;34(11):2101-6. doi: 10.1007/s10529-012-1014-4. Epub 2012 Jul 25.
Glucansucrase from Leuconostoc mesenteroides was immobilized in 1 % (w/v) with sodium alginate to produce oligosaccharides. Glucansucrase gave three activity bands of approx. 240, 178, and 165 kDa after periodic acid-Schiff staining with sucrose. The immobilized enzyme had 40 % activity after ten batch reactions at 30 °C and 75 % activity after a month of storage at 4 °C, which is six times more stable than the free enzyme. Immobilized enzyme was more stable at lower (3.5-4.5) and higher (6.5-7.0) pH ranges and higher temperatures (35-40 °C) compared with the free enzyme. Immobilized and free glucansucrase were employed in the acceptor reaction with maltose and each produced gluco-oligosaccharide ranging from trisaccharides to homologous pentasaccharides.
从肠膜明串珠菌中固定化的葡聚糖蔗糖酶（1%（w/v）的海藻酸钠）用于生产低聚糖。经 periodic acid-Schiff 染色与蔗糖反应后，葡聚糖蔗糖酶呈现出约 240、178 和 165 kDa 的三条活性带。固定化酶在 30°C 下进行十批次反应后具有 40%的活性，在 4°C 下储存一个月后仍具有 75%的活性，稳定性比游离酶高六倍。与游离酶相比，固定化酶在较低（3.5-4.5）和较高（6.5-7.0）pH 值范围以及较高温度（35-40°C）下更稳定。固定化和游离的葡聚糖蔗糖酶均用于与麦芽糖的受体反应，均可产生从三糖到同源五糖的葡萄糖低聚糖。
