Ul Qader Shah Ali, Aman Afsheen, Syed Noman, Bano Saeeda, Azhar Abid
Pharmaceutical Research Centre, PCSIR Laboratories complex, Karachi, Pakistan.
Ital J Biochem. 2007 Jun;56(2):158-62.
Immobilization of dextransucrase from Leuconostoc mesenteroides PCSIR-4 on alginate is optimized for application in the production of dextran from sucrose. Dextransucrase was partially purified by ethanol upto 2.5 fold. Properties of dextransucrase were less affected by immobilization on alginate beads from soluble enzyme. Highest activities of both soluble and immobilized dextransucrase found to be at 35 degrees C and optimum pH for activity remain 5.00. Substrate maxima for immobilized enzyme changed from 125 mg/ml to 200 mg/ml. Incubation time for enzyme-substrate reaction for maximum enzyme activity was increased from 15 minutes to 60 minutes in case of immobilized enzyme. Maximum stability of immobilized dextransucrase was achieved at 25 degrees C with respect to time.
对来自肠系膜明串珠菌PCSIR - 4的葡聚糖蔗糖酶固定在藻酸盐上进行了优化，以用于从蔗糖生产葡聚糖。葡聚糖蔗糖酶通过乙醇部分纯化至2.5倍。与可溶性酶相比，固定在藻酸盐珠上的葡聚糖蔗糖酶的性质受影响较小。可溶性和固定化葡聚糖蔗糖酶的最高活性均在35℃，最佳活性pH仍为5.00。固定化酶的底物最大值从125 mg/ml变为200 mg/ml。对于固定化酶，酶 - 底物反应达到最大酶活性的孵育时间从15分钟增加到60分钟。就时间而言，固定化葡聚糖蔗糖酶在25℃时达到最大稳定性。
