Department of Medicine, University of Padua, Via Giustiniani 2, Italy.
Exp Biol Med (Maywood). 2012 Jul;237(7):793-802. doi: 10.1258/ebm.2012.012024. Epub 2012 Jul 24.
SERPINB3 is a serine protease inhibitor with pleiotropic functions. It is involved in several physiological and pathological processes, where it appears to exert antiapoptotic effects. Little is known about its expression on immune system cells, the major players in mechanisms of viral defense and autoimmune disorders. The aim of this study was to characterize the expression of SERPINB3 on the surface of peripheral blood mononuclear cell subsets in both normal subjects and in patients with chronic viral infections and autoimmune diseases. Sixty-two patients were analyzed by flow cytometric analysis, including 45 with hepatitis C virus (HCV)-related chronic liver disease and 17 with systemic lupus erythematosus (SLE). SERPINB3 was expressed on B lymphocytes in 79% of the controls, in 32% of the HCV-infected patients and in none of the SLE patients. Surface localization of SERPINB3 was confirmed by confocal microscopy. SERPINB3 positivity was associated with CD27 reactivity (r = 0.98), but not to other activation molecules (CD69, CD71, CD86 and CXCR3). SERPINB3 is physiologically expressed on the surface of CD27(+) B lymphocytes, but its expression is reduced in HCV viral infection and not detectable in SLE patients. These results may suggest a role for SERPINB3 in B-cell defects typically found in viral infections and autoimmune disorders.
丝氨酸蛋白酶抑制剂 3（SERPINB3）是一种具有多种功能的丝氨酸蛋白酶抑制剂。它参与了许多生理和病理过程，在这些过程中，它似乎发挥了抗凋亡作用。关于其在免疫系统细胞（即病毒防御和自身免疫疾病机制中的主要参与者）上的表达知之甚少。本研究的目的是描述 SERPINB3 在正常人和慢性病毒感染及自身免疫性疾病患者外周血单个核细胞亚群表面的表达情况。通过流式细胞术分析了 62 例患者，包括 45 例丙型肝炎病毒（HCV）相关慢性肝病患者和 17 例系统性红斑狼疮（SLE）患者。在 79%的对照组、32%的 HCV 感染患者和无 SLE 患者中，SERPINB3 表达于 B 淋巴细胞表面。通过共聚焦显微镜证实了 SERPINB3 的表面定位。SERPINB3 阳性与 CD27 反应性相关（r=0.98），但与其他激活分子（CD69、CD71、CD86 和 CXCR3）无关。SERPINB3 生理性地表达于 CD27（+）B 淋巴细胞表面，但在 HCV 病毒感染中表达减少，在 SLE 患者中无法检测到。这些结果可能表明 SERPINB3 在病毒感染和自身免疫性疾病中常见的 B 细胞缺陷中发挥作用。
