Emmanuel College, Boston, Massachusetts 02115, United States.
Langmuir. 2012 Aug 21;28(33):12151-8. doi: 10.1021/la300949y. Epub 2012 Aug 7.
A quartz crystal microbalance (QCM) sensor was developed for the quantitation of calcium phosphate mineralization and the assessment of DNA as a template molecule. Inherent advantages of QCM, such as nanogram sensitivity, temporal resolution, surface-based measurements, and flow capabilities, were leveraged in the design of this sensor, and in-line fluidic mixing was used to control precursor reaction. This research shows that DNA, a highly programmable anionic polymer, is able to template and control mineralization of calcium phosphate, with nucleation occurring in less than 15 min and initial rates ranging from 4 to 8 ng/min. FT-IR measurements show mineralized material to be calcium phosphate resembling hydroxyapatite (HAP) when a DNA template is used. DNA is a promising mineralization template, and the QCM proves to be a dynamic technique for a broad range of heterogeneous mineralization experiments in complement to classic, diffusion-limited, end-point analysis techniques.
石英晶体微天平（QCM）传感器用于定量磷酸钙矿化和评估 DNA 作为模板分子。QCM 的固有优势，如纳克灵敏度、时间分辨率、基于表面的测量和流动能力，在该传感器的设计中得到了利用，并采用在线流体混合来控制前体反应。这项研究表明，DNA 作为一种高度可编程的阴离子聚合物，能够模板化和控制磷酸钙的矿化，成核发生在不到 15 分钟内，初始速率范围从 4 到 8 ng/min。傅里叶变换红外（FT-IR）测量表明，当使用 DNA 模板时，矿化材料是类似于羟磷灰石（HAP）的磷酸钙。DNA 是一种很有前途的矿化模板，而 QCM 被证明是一种动态技术，可用于广泛的非均相矿化实验，补充了经典的、扩散限制的、终点分析技术。
