Kim Francesca, Chen Tong, Burgess Trevor, Rasie Prakash, Selinger Tim Luca, Greschner Andrea, Rizis Georgios, Carneiro Karina
Faculty of Dentistry , University of Toronto , Toronto , ON M5G 1G6 , Canada . Email:
Institut National de la Recherche Scientifique (INRS) , EMT Research Center , Varennes , QC J3X 1S2 , Canada.
Chem Sci. 2019 Sep 27;10(45):10537-10542. doi: 10.1039/c9sc02811k. eCollection 2019 Dec 7.
The field of DNA nanotechnology uses synthetic DNA strands as building blocks for designing complex shapes in one-, two- and three-dimensions. Here, we investigate whether DNA nanostructures are feasible platforms for the precise organization of polyaspartic acid (pAsp), a known mineral carrier, with a goal towards biomimetic mineralization for enamel regeneration. We describe the preparation of DNA-pAsp conjugates and their subsequent assembly into ordered nanostructures. Covalent attachment of pAsp to DNA was noted to hinder DNA nanostructure formation past a certain threshold (50% pAsp) when tested on a previously published DNA system. However, a simplified double stranded DNA system (3sDH system) was more robust and efficient in its pAsp incorporation. In addition, the 3sDH system was successful in organizing mineral inducing groups in one dimension at repeating intervals of 28.7 ± 4.0 nm, as determined by atomic force microscopy. Our results demonstrate that DNA nanostructures can be functionalized with pAsp and act as a platform to investigate guided mineralization.
DNA纳米技术领域使用合成DNA链作为构建模块，用于设计一维、二维和三维的复杂形状。在此，我们研究DNA纳米结构是否是用于精确组织聚天冬氨酸（pAsp，一种已知的矿物质载体）的可行平台，目标是实现用于牙釉质再生的仿生矿化。我们描述了DNA-pAsp缀合物的制备及其随后组装成有序纳米结构的过程。当在先前发表的DNA系统上进行测试时，发现pAsp与DNA的共价连接在超过一定阈值（50% pAsp）时会阻碍DNA纳米结构的形成。然而，一种简化的双链DNA系统（3sDH系统）在掺入pAsp方面更加强健和高效。此外，通过原子力显微镜测定，3sDH系统成功地以28.7±4.0 nm的重复间隔在一维上组织了矿物诱导基团。我们的结果表明，DNA纳米结构可以用pAsp进行功能化，并作为研究引导矿化的平台。
