Tesch H, Fürbass R, Casper J, Lyons J, Bartram C R, Schmoll H J, Bronson D L
Medizinische Klinik, Universität Köln, FRG.
Int J Androl. 1990 Oct;13(5):377-88. doi: 10.1111/j.1365-2605.1990.tb01046.x.
We have analysed, by Northern blots, the expression of 14 cellular oncogenes in nine cell lines established from human teratocarcinomas. All lines expressed considerable amounts of p53, c-Ki-ras2, c-Ha-ras1, c-raf1, N-myc, and c-fos. Low level expression of c-myc was detected in some lines. Southern blot experiments revealed no amplification or rearrangement of the c-Ki-ras2, N-myc or c-fos genes. Using a rapid dot-blot screening procedure, based on a combination of in-vitro amplification of ras-specific sequences and oligonucleotide hybridization, we could detect no activation of Ha-ras or Ki-ras or any unexpressed N-ras sequences secondary to a point mutation at codons 12, 13, or 61.
