Tobaly-Tapiero J, Saal F, Peries J, Emanoil-Ravier R
Biochimie. 1986 Jul-Aug;68(7-8):1019-23. doi: 10.1016/s0300-9084(86)80045-1.
The structure of the ras gene family was analyzed in two human teratocarcinoma-derived cell lines, Tera I and Tera II, by DNA restriction enzyme digestion and Southern blot. We report here a ten-fold amplification of the c-Ki-ras-2 gene in these cell lines, whereas no structural alterations seem to occur either in c-Ha-ras-1 or N-ras. We also provide evidence indicating that no point mutation at codon 12, specifically recognized by Sac I, was detected. Moreover, DNA rearrangement, due to the loss of a Pvu II site located in the intervening sequences between the third and the fourth exon, has been found in both Tera I and Tera II.
通过DNA限制性内切酶消化和Southern印迹法，对两个人类畸胎瘤衍生细胞系Tera I和Tera II中的ras基因家族结构进行了分析。我们在此报告，在这些细胞系中c-Ki-ras-2基因有10倍的扩增，而c-Ha-ras-1或N-ras似乎没有发生结构改变。我们还提供了证据表明，未检测到由Sac I特异性识别的密码子12处的点突变。此外，在Tera I和Tera II中均发现了由于位于第三和第四外显子之间的间隔序列中一个Pvu II位点缺失而导致的DNA重排。
