Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
Comp Immunol Microbiol Infect Dis. 2012 Dec;35(6):575-81. doi: 10.1016/j.cimid.2012.07.001. Epub 2012 Jul 24.
We report the first description of Bartonella prevalence and genetic diversity in 64 Honshu sika deer (Cervus nippon centralis) and 18 Yezo sika deer (Cervus nippon yesoensis) in Japan. Overall, Bartonella bacteremia prevalence was 41.5% (34/82). The prevalence in wild deer parasitized with ticks and deer keds was 61.8% (34/55), whereas no isolates were detected in captive deer (0/27) free of ectoparasites. The isolates belonged to 11 genogroups based on a combination of the gltA and rpoB gene sequences. Phylogenetic analysis of concatenated sequences of the ftsZ, gltA, ribC, and rpoB genes of 11 representative isolates showed that Japanese sika deer harbor three Bartonella species, including B. capreoli and two novel Bartonella species. All Yezo deer's isolates were identical to B. capreoli B28980 strain isolated from an elk in the USA, based on the sequences of the ftsZ, gltA, and rpoB genes. In contrast, the isolates from Honshu deer showed a higher genetic diversity.
我们报告了日本 64 只本州梅花鹿（Cervus nippon centralis）和 18 只虾夷梅花鹿（Cervus nippon yesoensis）中 Bartonella 流行率和遗传多样性的首次描述。总体而言，Bartonella 菌血症的流行率为 41.5%（34/82）。在被蜱和鹿虱寄生的野生鹿中，流行率为 61.8%（34/55），而没有寄生体外寄生虫的圈养鹿（0/27）中未检测到分离株。根据 gltA 和 rpoB 基因序列的组合，分离株属于 11 个基因群。11 个代表性分离株的 ftsZ、gltA、ribC 和 rpoB 基因串联序列的系统发育分析表明，日本梅花鹿携带三种 Bartonella 物种，包括 B. capreoli 和两种新型 Bartonella 物种。根据 ftsZ、gltA 和 rpoB 基因序列，所有虾夷鹿的分离株均与从美国麋鹿中分离出的 B. capreoli B28980 株相同。相比之下，来自本州鹿的分离株显示出更高的遗传多样性。
