Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland.
Org Biomol Chem. 2012 Nov 21;10(43):8570-4. doi: 10.1039/c2ob26060c.
Analogues of the eukaryotic messenger RNA 5' end (m(7)G cap) are useful tools for studying mRNA fate and serve as reagents for in vitro preparation of 5' capped mRNAs. We designed a biotin-labeled dinucleotide cap analogue that can be incorporated into transcripts to produce 5'-capped and biotinylated mRNAs which retain their biological functionality and may be employed for biotin-(strept)avidin technologies.
真核生物信使 RNA 5' 端（m(7)G 帽）类似物是研究 mRNA 命运的有用工具，并且可作为体外制备 5' 加帽 mRNA 的试剂。我们设计了一种生物素标记的二核苷酸帽类似物，可将其掺入转录本中以产生 5'-加帽和生物素化的 mRNA，这些 mRNA 保留其生物学功能并且可用于生物素-(链霉)亲和素技术。
