Shao Wenxiang, Jin Hua, Huang Jie, Qiu Bin, Xia Ruihua, Deng Zeyuan, Cai Jiye, Chen Yong
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.
Scanning. 2013 Mar-Apr;35(2):119-26. doi: 10.1002/sca.21040. Epub 2012 Jul 25.
The integrity and adhesion properties of endothelium play vital roles during atherosclerosis. It is well known that oxidized low-density lipoprotein (Ox-LDL) influences many physiological activities or mechanical properties of endothelial cells. However, the effects of Ox-LDL on the integrity and nonspecific adhesion properties of endothelial cells are still unclear. In this study, using the topographical imaging and force measurement functions of atomic force microscopy (AFM), we found that Ox-LDL can transiently weaken the integrity of endothelium by impairing cell spreading of endothelial cells and decrease the attachment of irrelevant blood cells to endothelium by impairing the nonspecific adhesion property of endothelial cells. The AFM-based data provide important information for understanding the effects of Ox-LDL on endothelial cells or during atherogenesis.
内皮的完整性和黏附特性在动脉粥样硬化过程中起着至关重要的作用。众所周知，氧化型低密度脂蛋白（Ox-LDL）会影响内皮细胞的许多生理活动或力学特性。然而，Ox-LDL对内皮细胞完整性和非特异性黏附特性的影响仍不清楚。在本研究中，我们利用原子力显微镜（AFM）的形貌成像和力测量功能，发现Ox-LDL可通过损害内皮细胞的铺展而暂时削弱内皮的完整性，并通过损害内皮细胞的非特异性黏附特性而减少无关血细胞与内皮的附着。基于AFM的数据为理解Ox-LDL对内皮细胞的影响或动脉粥样硬化发生过程提供了重要信息。
