Liu Y, Yu S L
Department of Applied Chemistry, Beijing Institute of Chemical Technology, China.
J Chromatogr. 1990 Aug 31;515:169-73. doi: 10.1016/s0021-9673(01)89310-9.
Styrene-divinylbenzene copolymer 60-80 mesh was used as the matrix and ground to a reasonable particle size in a colloid mill. Small and uniform particles (5-15 microns) can be obtained in a laboratory-made continuous acetone elutriation device. After elutriation the iminodiacetic acid (IDA) chelating resin was synthesized, and the infrared spectrum of the product indicated that the synthetic reaction is effective. The Cu(II)-modified IDA chelating resin was used as a stationary phase in high-performance immobilized metal ion affinity chromatography, packed into a column by the high-pressure slurry-packing technique. Methanol, to which ammonia was added as a competitive ligand, was used as the mobile phase for the separation of aromatic amines. The retention value of aromatic amines depends mainly on their basicity and molecular size. The results showed that some aromatic amines can be separated with the Cu(II)-IDA column.
采用60 - 80目苯乙烯 - 二乙烯基苯共聚物作为基质，并在胶体磨中研磨至合理粒径。在实验室自制的连续丙酮淘析装置中可获得小而均匀的颗粒（5 - 15微米）。淘析后合成亚氨基二乙酸（IDA）螯合树脂，产物的红外光谱表明合成反应有效。将Cu(II)修饰的IDA螯合树脂用作高效固定化金属离子亲和色谱中的固定相，通过高压匀浆填充技术填充到柱中。添加氨作为竞争配体的甲醇用作分离芳香胺的流动相。芳香胺的保留值主要取决于它们的碱性和分子大小。结果表明，某些芳香胺可用Cu(II)-IDA柱进行分离。
