Nishi H, Fukuyama T, Matsuo M, Terabe S
Analytical Chemistry Research Laboratory, Tanabe Seiyaku, Co., Ltd., Osaka, Japan.
J Chromatogr. 1990 Aug 31;515:233-43. doi: 10.1016/s0021-9673(01)89318-3.
The separation of optically isomeric diltiazem hydrochloride, trimetoquinol hydrochloride and related compounds by micellar electrokinetic chromatography was investigated employing four bile salts as chiral surfactants. The chiral separation of diltiazem hydrochloride and timetoquinol hydrochloride was successfully achieved by use of sodium taurodeoxycholate under neutral conditions, although enantiomers of carboline derivatives A and B and 2,2'-dihydroxy-1,1'-dinaphthyl were resolved with all the bile salts under conditions from neutral to alkaline. The chiral separation of diltiazem-related compounds was affected by the structure of the samples in addition to the effects of bile salt structures and pH of the buffer solutions. Application to the optical purity testing of trimetoquinol hydrochloride by the area percentage method is described. A possible chiral separation mechanism is briefly mentioned.
采用四种胆汁盐作为手性表面活性剂，研究了胶束电动色谱法分离光学异构体盐酸地尔硫䓬、盐酸曲美喹诺及相关化合物。在中性条件下，使用牛磺去氧胆酸钠成功实现了盐酸地尔硫䓬和盐酸曲美喹诺的手性分离，尽管在中性至碱性条件下，所有胆汁盐都能拆分咔啉衍生物A和B以及2,2'-二羟基-1,1'-联萘的对映体。除了胆汁盐结构和缓冲溶液pH值的影响外，地尔硫䓬相关化合物的手性分离还受样品结构的影响。描述了用面积百分比法应用于盐酸曲美喹诺光学纯度检测。简要提及了一种可能的手性分离机制。
