Noggle F T, Clark C R, DeRuiter J
Alabama Department of Forensic Sciences, Wire Road, Auburn 36830.
J Chromatogr Sci. 1990 May;28(5):263-8. doi: 10.1093/chromsci/28.5.263.
The liquid chromatographic separation of the common anabolic steroid esters is accomplished in an isocratic reversed-phase system. These anabolic steroids are now controlled substances in many states in the U.S. due to their potential for abuse and misuse. The esters are designed to be very lipophilic materials for slow release from intramuscular injection sites. With a C18 stationary phase material and a mobile phase of methanol-water (85:15), the more lipophilic esters such as testosterone decanoate show a retention time of about 50 min. These esters of varying lipophilicity are well resolved by reversed-phase liquid chromatography, and this technique is very useful for the identification of these compounds in forensic samples.
常见合成代谢类固醇酯的液相色谱分离是在等度反相系统中完成的。由于这些合成代谢类固醇有被滥用和误用的可能性，它们现在在美国许多州都是受控物质。这些酯被设计成非常亲脂性的材料，以便从肌肉注射部位缓慢释放。使用C18固定相材料和甲醇 - 水（85:15）的流动相，癸酸睾酮等亲脂性更强的酯的保留时间约为50分钟。这些亲脂性不同的酯通过反相液相色谱法能得到很好的分离，并且该技术对于法医样本中这些化合物的鉴定非常有用。
