Noggle F T, Clark C R, DeRuiter J
Alabama Department of Forensic Sciences, Auburn 36830.
J Chromatogr Sci. 1990 Apr;28(4):162-6. doi: 10.1093/chromsci/28.4.162.
The liquid chromatographic properties of various 17-hydroxy anabolic steroids are examined under reversed-phase conditions. These anabolic steroids are now listed as controlled drugs in many states due to their abuse potential in athletics, body building, and other areas. These nonesterified steroids are separated on a C18 stationary phase with a 70% methanol in water mobile phase. In a few cases, two compounds display very similar retention properties. However, dual-wavelength detection at 254 and 280 nm allows for their differentiation. Reversed-phase retention parallels steroid lipophilicity based on hydroxyl and methyl group substituents. Also, those steroids containing a dienone substructure are more polar than steroids containing an enone moiety.
在反相条件下研究了各种17-羟基合成代谢类固醇的液相色谱性质。由于这些合成代谢类固醇在体育、健身及其他领域存在滥用可能性，目前在许多州被列为管制药物。这些非酯化类固醇在C18固定相上进行分离，流动相为70%甲醇的水溶液。在少数情况下，两种化合物表现出非常相似的保留特性。然而，在254和280nm处的双波长检测可实现它们的区分。基于羟基和甲基取代基，反相保留与类固醇亲脂性平行。此外，含有二烯酮亚结构的类固醇比含有烯酮部分的类固醇极性更强。
