Katnik I, Dobryszycka W
Department of Biochemistry, Medical Academy, Wrocław, Poland.
J Immunoassay. 1990;11(4):503-17. doi: 10.1080/01971529008055047.
A novel immunoenzymatic assay is described for the quantitation of human haptoglobin (Hp). Two binding sites on the Hp molecule, namely for hemoglobin (Hb) and for the specific antibody, are involved in the reaction. Hb adsorbed onto the polystyrene microplate binds Hp present in any biological fluid. The formed Hp-Hb complex is detected with horse-radish peroxidase conjugated with anti-Hp antibody. By means of this ELISA, Hp may be measured in the range of 5 to 150 micrograms/L. Comparison of the Hp-ELISA with two other methods of Hp determination resulted in correlation coefficients of 0.97 to 0.99. Intra- and inter-assay coefficients of variation ranged from 4.7 to 6.7%. Hp levels were measured in urine, cord serum, cerebrospinal fluid, amniotic fluid and saliva.
本文描述了一种用于定量检测人触珠蛋白（Hp）的新型免疫酶测定法。该反应涉及Hp分子上的两个结合位点，即与血红蛋白（Hb）结合的位点和与特异性抗体结合的位点。吸附在聚苯乙烯微孔板上的Hb可结合任何生物流体中存在的Hp。用与抗Hp抗体偶联的辣根过氧化物酶检测形成的Hp-Hb复合物。通过这种酶联免疫吸附测定法（ELISA），可在5至150微克/升的范围内检测Hp。将Hp-ELISA与另外两种Hp测定方法进行比较，相关系数为0.97至0.99。批内和批间变异系数范围为4.7%至6.7%。对尿液、脐带血清、脑脊液、羊水和唾液中的Hp水平进行了检测。
