Katnik I, Dobryszycka W
Department of Biochemistry, Medical Academy, Wrocław, Poland.
J Immunoassay. 1992;13(2):145-62. doi: 10.1080/15321819208021224.
Two-site lectin-haptoglobin-enzyme immunosorbent assay (L-Hp-ELISA), is described. Haptoglobin binding to Concanavalin A, immobilized to polystyrene microtiter plate, was estimated by anti-haptoglobin polyclonal and monoclonal antibodies conjugated with horse-radish peroxidase. The range of haptoglobin binding to Concanavalin A, measured by the L-Hp-ELISA was 25 to 300 ng/ml using polyclonal, and 50 to 600 ng/ml using monoclonal anti-haptoglobin antibodies, respectively.
描述了双位点凝集素 - 触珠蛋白 - 酶免疫吸附测定法（L-Hp-ELISA）。通过与辣根过氧化物酶偶联的抗触珠蛋白多克隆和单克隆抗体，估计固定在聚苯乙烯微量滴定板上的伴刀豆球蛋白A与触珠蛋白的结合情况。使用多克隆抗体时，通过L-Hp-ELISA测定的触珠蛋白与伴刀豆球蛋白A的结合范围为25至300 ng/ml，使用单克隆抗触珠蛋白抗体时为50至600 ng/ml。
