使用多克隆抗体和单克隆抗体开发用于糖化触珠蛋白的伴刀豆球蛋白A-酶免疫吸附测定法。

Development of concanavalin A-enzyme immunosorbent assay for glycated haptoglobin using polyclonal and monoclonal antibodies.

作者信息

Katnik I, Dobryszycka W

机构信息

Department of Biochemistry, Medical Academy, Wrocław, Poland.

出版信息

J Immunoassay. 1992;13(2):145-62. doi: 10.1080/15321819208021224.

DOI:10.1080/15321819208021224

PMID:1430237

Abstract

Two-site lectin-haptoglobin-enzyme immunosorbent assay (L-Hp-ELISA), is described. Haptoglobin binding to Concanavalin A, immobilized to polystyrene microtiter plate, was estimated by anti-haptoglobin polyclonal and monoclonal antibodies conjugated with horse-radish peroxidase. The range of haptoglobin binding to Concanavalin A, measured by the L-Hp-ELISA was 25 to 300 ng/ml using polyclonal, and 50 to 600 ng/ml using monoclonal anti-haptoglobin antibodies, respectively.

摘要

描述了双位点凝集素 - 触珠蛋白 - 酶免疫吸附测定法（L-Hp-ELISA）。通过与辣根过氧化物酶偶联的抗触珠蛋白多克隆和单克隆抗体，估计固定在聚苯乙烯微量滴定板上的伴刀豆球蛋白A与触珠蛋白的结合情况。使用多克隆抗体时，通过L-Hp-ELISA测定的触珠蛋白与伴刀豆球蛋白A的结合范围为25至300 ng/ml，使用单克隆抗触珠蛋白抗体时为50至600 ng/ml。