Zelman M E, Lange C F
Loyola University Chicago, Department of Microbiology, Maywood, IL 60153.
J Immunoassay. 1990;11(4):545-54. doi: 10.1080/01971529008055049.
Solid-phase C1q was used to remove antigen/antibody complexes in an inhibition ELISA for low molecular weight streptococcal cell membrane (SCM) polypeptide antigens. To selectively fix IgM monoclonal antibody bound to antigen, binding was carried out in C1q-coated ELISA plates; transfer of supernatants to SCM-coated plates for ELISA permitted measurement of residual antibody. When inhibition occurred in the presence of C1q, the maximal binding was 72-98%. In the absence of C1q the maximum apparent binding was only 45-50%, which we attribute to displacement of the initially bound SCM antigen by solid phase SCM antigen. Removal of antigen/antibody complexes by solid-phase Clq during inhibition assays may facilitate analysis of low affinity antigen/antibody interactions.
在用于低分子量链球菌细胞膜（SCM）多肽抗原的抑制性酶联免疫吸附测定（ELISA）中，使用固相C1q去除抗原/抗体复合物。为了选择性固定与抗原结合的IgM单克隆抗体，结合反应在包被C1q的ELISA板中进行；将上清液转移至包被SCM的板中进行ELISA，从而能够测量残留抗体。当在C1q存在的情况下发生抑制时，最大结合率为72%-98%。在不存在C1q的情况下，最大表观结合率仅为45%-50%，我们将其归因于固相SCM抗原取代了最初结合的SCM抗原。在抑制试验期间通过固相Clq去除抗原/抗体复合物可能有助于分析低亲和力抗原/抗体相互作用。
