Nuwal Nidhi, Stock Patrick, Hiemeyer Jochen, Schmid Benjamin, Fiala André, Buchner Erich
Theodor-Boveri Institute, Department of Genetics and Neurobiology, University of Würzburg, Würzburg, Germany.
J Neurogenet. 2012 Sep;26(3-4):298-305. doi: 10.3109/01677063.2012.700266. Epub 2012 Jul 27.
Animals have to perform adequate behavioral actions dependent on internal states and environmental situations, and adjust their behavior according to positive or negative consequences. The fruit fly Drosophila melanogaster represents a key model organism for the investigation of neuronal mechanisms underlying adaptive behavior. The authors are using a behavioral paradigm in which fruit flies attached to a manipulator can walk on a Styrofoam ball whose movements are recorded such that intended left or right turns of the flies can be registered and used to operantly control heat stimuli or optogenetic activation of distinct subsets of neurons. As proof of principle, the authors find that flies in this situation avoid heat stimuli but prefer optogenetic self-stimulation of sugar receptors. Using this setup it now should be possible to study the neuronal network underlying positive and negative value assessment of adult Drosophila in an operant setting.
动物必须根据内部状态和环境情况执行适当的行为动作，并根据积极或消极后果调整其行为。果蝇是研究适应性行为背后神经元机制的关键模式生物。作者正在使用一种行为范式，其中附着在操纵器上的果蝇可以在一个聚苯乙烯泡沫塑料球上行走，该球的运动被记录下来，这样果蝇预期的向左或向右转就可以被记录下来，并用于操作性地控制热刺激或对不同神经元子集的光遗传学激活。作为原理证明，作者发现处于这种情况下的果蝇会避开热刺激，但更喜欢对糖受体进行光遗传学自我刺激。利用这种设置，现在应该有可能在操作性环境中研究成年果蝇正负价值评估背后的神经网络。
