Grigolato P, Benetti A, Berenzi A, Villanacci V, Tardanico R
Institute of Anatomy and Histological Pathology, University of Brescia, Italy.
Int J Biol Markers. 1990 Apr-Jun;5(2):81-4.
We examined 35 cases of stomach carcinoma and 40 cases of colonic carcinoma with PNA associated with peroxidase (peanut agglutinin, lectin which binds to the terminal disaccharide galactose beta (1,3)-N-acetil-galacto-samine). In this way evaluation of the functional aspects of the normal-neoplastic sequence was undertaken. This method was carried out for histological and ultrastructural investigations. The results obtained in both cases showed a different reactivity in the evolution of neoplastic disease: in fact, positivity in dysplasia is finely granular intracytoplasmic, whereas in well-differentiated neoplastic transformation such a reactivity is preferentially localized along the cellular membranes, with restoration of gross positivity in the cytoplasm for the poorly-differentiated neoplasm. We therefore believe PNA to be a marker not only of neoplastic progression but of differentiation as well: we also hypothesize it to reveal glycoprotein groups with possible antigenic power, involved in immunologic interactions between tumor and host.
我们用与过氧化物酶相关的PNA（花生凝集素，一种能与末端二糖半乳糖β(1,3)-N-乙酰半乳糖胺结合的凝集素）检测了35例胃癌和40例结肠癌。通过这种方式对正常-肿瘤序列的功能方面进行了评估。该方法用于组织学和超微结构研究。在这两种病例中获得的结果显示，在肿瘤疾病的发展过程中存在不同的反应性：事实上，发育异常中的阳性表现为细颗粒状胞质内阳性，而在高分化肿瘤转化中，这种反应性优先定位于细胞膜，低分化肿瘤的细胞质中则恢复为总体阳性。因此，我们认为PNA不仅是肿瘤进展的标志物，也是分化的标志物：我们还推测它能揭示具有可能抗原性的糖蛋白基团，这些基团参与肿瘤与宿主之间的免疫相互作用。
