Bargielowski Irka, Kaufmann Christian, Alphey Luke, Reiter Paul, Koella Jacob
Division of Biology, Faculty of Natural Sciences, Imperial College London , London, United Kingdom.
Vector Borne Zoonotic Dis. 2012 Dec;12(12):1053-8. doi: 10.1089/vbz.2012.0994. Epub 2012 Jul 26.
The ability of sterile males to survive, disperse, find, and mate with wild females is key to the success of sterile insect technique (SIT). The Release of Insects carrying a Dominant Lethal (RIDL) system is a genetics-based SIT strategy for Aedes aegypti. We examine two aspects of insect performance, flight potential (dispersal ability) and teneral energy reserves, by comparing wild-type (WT) males with genetically-modified lines carrying the tetracycline-repressible constructs OX513A and OX3604C. Our results show significant differences in the flight capacity of the modified lines. OX513A males bred with tetracycline covered 38% less distance, while OX3604C males reared without tetracycline spent 21% less time in flight than their WT counterparts. Such differences in flight performance should be considered when designing release programs (e.g., by placing release sites sufficiently close together to achieve adequate coverage). All mosquito lines had similar teneral carbohydrate contents, though males of the OX3604C line contained more lipids. The addition of tetracycline to the larval diet did not influence the flight potential of the males; however, it did change the teneral sugar reserves of the WT and the lipid reserves of both the WT and the OX3604C lines.
无菌雄蚊的生存、扩散、寻找野生雌蚊并与之交配的能力是昆虫不育技术（SIT）成功的关键。携带显性致死基因的昆虫释放（RIDL）系统是一种基于遗传学的埃及伊蚊不育昆虫技术策略。我们通过将野生型（WT）雄蚊与携带四环素可抑制构建体OX513A和OX3604C的转基因品系进行比较，研究了昆虫性能的两个方面，即飞行潜力（扩散能力）和羽化时的能量储备。我们的结果显示，转基因品系的飞行能力存在显著差异。用四环素饲养的OX513A雄蚊飞行距离比野生型雄蚊少38%，而未用四环素饲养的OX3604C雄蚊飞行时间比野生型雄蚊少21%。在设计释放计划时（例如，通过将释放地点设置得足够靠近以实现充分覆盖），应考虑这种飞行性能的差异。所有蚊系羽化时的碳水化合物含量相似，不过OX3604C品系的雄蚊含有更多的脂质。在幼虫饲料中添加四环素不会影响雄蚊的飞行潜力；然而，它确实改变了野生型雄蚊羽化时的糖储备以及野生型和OX3604C品系雄蚊的脂质储备。
