Su Sha, Zhang Xiaohe, Jian Chengzhi, Huang Baojian, Peng Xiong, Vreysen Marc J B, Chen Maohua
Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China.
Joint FAO/IAEA Programme, Entomology Unit, FAO/IAEA Agriculture & Biotechnology Laboratory, International Atomic Energy Agency, A-2444 Vienna, Austria.
Insects. 2022 Aug 13;13(8):725. doi: 10.3390/insects13080725.
The oriental fruit moth, (Busck) (Lepidoptera: Tortricidae) is one of the most severe fruit tree pests in China, causing huge economic losses to fruit production. So far, there are few detailed reports on the rearing protocols of . In this study, we compared the longevity, 10-d fecundity, flight ability, and the activity of major energy metabolism enzymes in the flight muscles of under three feeding regimes (supplement with sterile water, supplement with 10% honey solution and starvation) of the adult moths. The results showed that the longevity, 10-d fecundity, and flight parameters (cumulative flight distance and time, maximum flight distance and duration, and the average flight speed) of adult moths when supplemented with sterile water or honey solution were significantly higher than those of moths that were starved. There were no significant differences in the 10-d fecundity, flight parameters, and the activity of major energy metabolism enzymes of flight muscles between moths that were supplemented with sterile water or 10% honey solution. The flight muscles of mainly used carbohydrates as an energy source when sterile water and honey solution were supplemented, and the moth mainly used lipids as an energy source under starvation. Considering the cost and potential for diet contamination during mass-rearing, supplying sterile water is considered a cost effective option for food substitution of adult .
梨小食心虫（Grapholita molesta (Busck)，鳞翅目：卷蛾科）是中国最严重的果树害虫之一，给水果生产造成巨大经济损失。迄今为止，关于梨小食心虫饲养方案的详细报道很少。在本研究中，我们比较了成年蛾在三种饲养方式（补充无菌水、补充10%蜂蜜溶液和饥饿）下的寿命、10天繁殖力、飞行能力以及飞行肌肉中主要能量代谢酶的活性。结果表明，补充无菌水或蜂蜜溶液时成年蛾的寿命、10天繁殖力和飞行参数（累计飞行距离和时间、最大飞行距离和持续时间以及平均飞行速度）显著高于饥饿处理的蛾。补充无菌水或10%蜂蜜溶液的蛾在10天繁殖力、飞行参数和飞行肌肉主要能量代谢酶活性方面没有显著差异。补充无菌水和蜂蜜溶液时，梨小食心虫的飞行肌肉主要利用碳水化合物作为能量来源，而在饥饿状态下，该蛾主要利用脂质作为能量来源。考虑到大规模饲养的成本和饮食污染的可能性，供应无菌水被认为是替代成年梨小食心虫食物的一种经济有效的选择。
