International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149, Trieste, Italy.
J Proteomics. 2012 Oct 22;75(18):5911-9. doi: 10.1016/j.jprot.2012.07.019. Epub 2012 Jul 24.
Xanthomonas oryzae pv. oryzae (Xoo) is the second most important rice pathogen, causing a disease called bacterial leaf blight. Xoo colonizes and infects the vascular tissue resulting in tissue necrosis and wilting causing significant yield losses worldwide. In this study Xoo infected vascular fluid (xylem sap) was recovered and analyzed for secreted Xoo proteins. Three independent experiments resulted in the identification of 324 different proteins, 64 proteins were found in all three samples which included many of the known virulence-associated factors. In addition, 10 genes encoding for the identified proteins were inactivated and one mutant displayed statistically a significant loss in virulence when compared to the wild type Xoo, suggesting that a new virulence-associated factor has been revealed. The usefulness of this approach in understanding the lifestyle and unraveling the virulence-associated factors of phytopathogenic vascular bacteria is discussed.
稻黄单胞菌（Xoo）是第二大重要的水稻病原体，引起细菌性叶斑病。Xoo 定殖并感染维管束组织，导致组织坏死和萎蔫，在全球范围内造成重大产量损失。在这项研究中，回收了感染的血管液（木质部汁液）并分析了分泌的 Xoo 蛋白。三个独立的实验鉴定出 324 种不同的蛋白质，在所有三个样本中发现了 64 种蛋白质，其中包括许多已知的与毒力相关的因子。此外，有 10 个编码鉴定出的蛋白质的基因被失活，与野生型 Xoo 相比，一个突变体在毒力方面表现出统计学上的显著下降，表明揭示了一个新的与毒力相关的因子。讨论了这种方法在理解植物病原性血管细菌的生活方式和揭示与毒力相关的因子方面的有用性。
