Dermatology Department, College of Medicine, Qassim University, Saudi Arabia.
J Dermatol Sci. 2012 Oct;68(1):52-5. doi: 10.1016/j.jdermsci.2012.07.001. Epub 2012 Jul 16.
Cytokines play an important role in the pathogenesis of acne vulgaris together with other genetic and environmental factors.
To check for the association of TNF-α and IL-10 gene polymorphisms with the susceptibility and severity of acne in Saudi patients.
Study subjects included 166 Saudi patients (65 males, 101 females) with acne vulgaris. Their mean age±SD was 21.6±5.1 years. These cases were compared to 390 unrelated healthy controls (208 males, 182 females) with a mean age±SD of 20.1±3.3 years. Cases were sub-grouped on the basis of their severity of acne affection into mild, moderate and severe groups. For all participants, genotypic variants of the TNF-α -308 G/A and IL-10 -1082 A/G genes were determined using the real time PCR technique.
Frequencies of genotypic variants of the TNF-α -308 polymorphism were significantly different in acne cases compared to controls. Further analysis showed that acne cases had significantly higher frequency of both the GG and AA homozygous forms than controls (73.8% vs. 63.6%, p=0.02, odds ratio=1.6). It was also interestingly noticed that the amount of GG homozygosity was notably higher among female cases than male ones (76.0% vs. 54.7%, p=0.006, odds ratio=2.6) whereas male cases had a higher frequency of AA and GA genotypes than female ones (9.4% and 35.9% vs. 4% and 20% respectively). Differences in the frequencies of IL-10 -1082 genotypic variants were statistically insignificant comparing cases to controls (p=0.3). On the other hand, comparing cases-subgroups in terms of the age of onset of the disease, consanguinity, family history, obesity and acne severity; no statistical significance was observed regarding frequencies of genotypic variants related to the both TNF-α -308 and IL-10 -1082 polymorphisms (>0.05).
TNF-α -308 polymorphic variants might be a predisposing factor for acne susceptibility, with no apparent relation to its severity whereas IL-10 -1082 variants showed no association with both acne susceptibility and severity.
细胞因子在寻常痤疮的发病机制中与其他遗传和环境因素一起发挥重要作用。
检测 TNF-α 和 IL-10 基因多态性与沙特患者痤疮易感性和严重程度的关系。
研究对象包括 166 例沙特寻常痤疮患者（男 65 例，女 101 例），平均年龄±标准差为 21.6±5.1 岁。将这些病例与 390 名无亲缘关系的健康对照者（男 208 名，女 182 名）进行比较，平均年龄±标准差为 20.1±3.3 岁。根据痤疮严重程度将病例分为轻度、中度和重度组。对所有参与者，采用实时 PCR 技术检测 TNF-α-308 G/A 和 IL-10-1082 A/G 基因的基因型变异。
与对照组相比，TNF-α-308 多态性的基因型变异频率在痤疮病例中明显不同。进一步分析表明，痤疮病例的 GG 和 AA 纯合形式的频率明显高于对照组（73.8%比 63.6%，p=0.02，优势比=1.6）。有趣的是，女性病例的 GG 纯合度明显高于男性病例（76.0%比 54.7%，p=0.006，优势比=2.6），而男性病例的 AA 和 GA 基因型频率高于女性病例（9.4%和 35.9%比 4%和 20%）。与对照组相比，IL-10-1082 基因型变异的频率差异无统计学意义（p=0.3）。另一方面，根据疾病发病年龄、近亲结婚、家族史、肥胖和痤疮严重程度对病例亚组进行比较，TNF-α-308 和 IL-10-1082 多态性相关基因型变异的频率无统计学意义（>0.05）。
TNF-α-308 多态性可能是痤疮易感性的一个易感因素，与疾病严重程度无明显关系，而 IL-10-1082 变异与痤疮易感性和严重程度均无关联。
