Farbiarz S R, De Carvalho T U, Alviano C, De Souza W
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
J Med Vet Mycol. 1990;28(5):373-83.
The interaction between conidia of Fonsecaea pedrosoi and mouse resident peritoneal macrophages was observed by light microscopy and by scanning (SEM) and transmission (TEM) electron microscopy. The conidia first attached to the surface of the macrophage and were then ingested. Prolonged incubation of the macrophage cultures showed proliferation of intracellular fungi as well as those which remained attached to the macrophage surface. The conidia were ingested by a typical phagocytic process, with formation of a phagosome. Macrophage lysosomes were observed to fuse with the phagosomes by immunofluorescence microscopy of macrophages previously labeled with acridine orange, by TEM of thin sections of macrophages labeled with albumin-gold, and by ultrastructural localization of acid phosphatase within the phagosomes.
通过光学显微镜、扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察了佩德罗霉分生孢子与小鼠驻留腹膜巨噬细胞之间的相互作用。分生孢子首先附着在巨噬细胞表面，然后被吞噬。巨噬细胞培养物的长时间孵育显示细胞内真菌以及那些仍附着在巨噬细胞表面的真菌增殖。分生孢子通过典型的吞噬过程被吞噬，形成吞噬体。通过用吖啶橙预先标记的巨噬细胞的免疫荧光显微镜、用白蛋白金标记的巨噬细胞薄片的TEM以及吞噬体内酸性磷酸酶的超微结构定位，观察到巨噬细胞溶酶体与吞噬体融合。
