Quintara Biosciences, Albany CA 9470, United States.
Methods. 2012 Dec;58(4):360-6. doi: 10.1016/j.ymeth.2012.07.017. Epub 2012 Jul 24.
High-throughput technologies such as affinity purification and yeast two-hybrid (Y2H) screening have been applied to explore protein-protein interactions (PPIs) in the model organism Saccharomyces cerevisiae. The "Cross-and-Capture" system is an alternative approach for an assessment of PPIs using two arrayed collections of differentially tagged yeast strains. In its current implementation, Cross-and-Capture encompasses ∼500 endogenously tagged yeast proteins, predominantly with roles in DNA metabolism and maintenance. The tagged arrays can also serve other purposes in yeast proteomics, such as monitoring of protein expression and the detection of posttranslational protein modifications. In this article, I summarize the development of this tool and describe its application to detect protein complexes and to screen for novel PPIs.
高通量技术，如亲和纯化和酵母双杂交（Y2H）筛选，已被应用于探索模式生物酿酒酵母中的蛋白质-蛋白质相互作用（PPIs）。“交叉捕获”系统是一种使用两种差异标记酵母菌株阵列评估 PPIs 的替代方法。在其当前的实现中，交叉捕获包含约 500 个内源性标记的酵母蛋白，主要作用于 DNA 代谢和维持。标记的阵列在酵母蛋白质组学中也可以用于其他目的，如监测蛋白质表达和检测翻译后蛋白质修饰。在本文中，我总结了该工具的发展，并描述了其在检测蛋白质复合物和筛选新的 PPIs 中的应用。
