Department of Paediatrics and Endocrinology, Medical University of Warsaw, Warsaw, Poland.
Adv Exp Med Biol. 2013;756:131-8. doi: 10.1007/978-94-007-4549-0_17.
Antibodies against the Na/I symporter (anti-NIS ab) have been found in adult patients with autoimmune thyroid diseases. As easily available for the immune system, NIS can play a role in the initial stage of autoimmune thyroid diseases. Children with Turner's syndrome (TS) being at high risk of autoimmune thyroid disease development seem a valuable group for the investigation of the early autoimmune process. The aim of the study was to investigate the presence of anti-NIS ab and its potential clinical significance in TS children. Fifty four girls with TS were examined (age 11.9 ± 2.46 years), and 23 healthy girls with normal thyroid function, free of autoimmune diseases. Anti-NIS antibodies were measured by the in-house ELISA method and the Western blotting. Sera considered positive for anti-NIS ab were used for the iodide uptake bioassay using COS7 cells stably transfected with hNIS. In all patients the thyroid function, antithyroid antibodies presence and thyroid ultrasonography were evaluated. In 20% of the patients a subclinical hypothyroidism was diagnosed and 70.4% had antithyroid antibodies (anti-TPO - 64.8% and Anti-Tg - 24%). Anti-NISab were present in 14.8% girls with TS and in none of the control group. Their presence was unrelated to other antithyroid antibodies titre or patients' age. A positive correlation between the anti-NIS ab presence and the hypothyroidism was found (p < 0.04). Anti-NIS ab-positive sera did not suppress iodine uptake. In conclusion, anti-NIS antibodies were present in 14.8% of children with TS and they were related to the presence of hypothyroidism.
针对钠/碘同向转运体（NIS）的抗体（抗-NIS ab）已在自身免疫性甲状腺疾病的成年患者中被发现。由于 NIS 易于被免疫系统识别，因此它可能在自身免疫性甲状腺疾病的初始阶段发挥作用。特纳综合征（TS）患儿发生自身免疫性甲状腺疾病的风险较高，因此他们似乎是研究早期自身免疫过程的有价值群体。本研究旨在调查 TS 儿童中抗-NIS ab 的存在及其潜在的临床意义。我们对 54 名 TS 女孩（年龄 11.9 ± 2.46 岁）进行了检查，同时还对 23 名甲状腺功能正常、无自身免疫性疾病的健康女孩进行了检查。我们通过内部 ELISA 法和 Western blot 法检测了抗-NIS ab。将被认为阳性的血清用于 COS7 细胞的碘摄取生物测定，该细胞稳定转染了 hNIS。在所有患者中，我们都评估了甲状腺功能、抗甲状腺抗体的存在和甲状腺超声检查。20%的患者被诊断为亚临床甲状腺功能减退症，70.4%的患者存在抗甲状腺抗体（抗 TPO - 64.8%和抗 Tg - 24%）。14.8%的 TS 女孩存在抗-NIS ab，而对照组中无一例存在。它们的存在与其他抗甲状腺抗体滴度或患者年龄无关。我们发现抗-NIS ab 的存在与甲状腺功能减退症呈正相关（p < 0.04）。阳性抗-NIS ab 血清并不能抑制碘摄取。总之，14.8%的 TS 儿童存在抗-NIS ab，它们与甲状腺功能减退症的存在有关。
