College of Life Sciences, South China Agriculture University, Guangzhou, People's Republic of China.
Am J Bot. 2012 Aug;99(8):e337-9. doi: 10.3732/ajb.1200065. Epub 2012 Jul 26.
Microsatellite markers were developed to characterize the level of genetic diversity and population genetic structure of Cercis chinensis, a widely cultivated garden plant in China with congeneric species disjunctly distributed in East Asia, North America, and the Mediterranean.
Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, eight microsatellite markers were developed in C. chinensis. Seven of the markers displayed polymorphism, with the number of alleles ranging from one to four in four populations of C. chinensis. Four to six microsatellite loci exhibited interspecific transferability in C. glabra, C. chuniana, and C. chingii.
These are the first microsatellite markers developed for C. chinensis, which will be further used in investigation of population genetic structure and phylogeographic pattern of C. chinensis and its congeneric species.
微卫星标记被开发出来，以描述中国广泛种植的园林植物紫荆的遗传多样性水平和种群遗传结构，其同属物种分布在东亚、北美和地中海地区。
使用序列中重复快速分离 AFLP（FIASCO）协议，在紫荆中开发了 8 个微卫星标记。其中 7 个标记显示多态性，在紫荆的四个种群中，等位基因数从一个到四个不等。在紫荆、紫荆和紫荆中，有 4 到 6 个微卫星位点具有种间可转移性。
这些是为紫荆开发的第一批微卫星标记，它们将进一步用于紫荆及其同属物种的种群遗传结构和系统地理学模式的研究。
