杜氏翅棱芹微卫星标记。
Microsatellite markers for Duperrea pavettifolia (Rubiaceae).
机构信息
Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Gardens, Chinese Academy of Sciences, Mengla, Yunnan, People's Republic of China.
出版信息
Am J Bot. 2012 Aug;99(8):e310-2. doi: 10.3732/ajb.1100609. Epub 2012 Jul 26.
PREMISE OF THE STUDY
The development of microsatellite primers for Duperrea pavettifolia will be the foundation for mating system analysis and conservation research.
METHODS AND RESULTS
Nineteen microsatellite markers were developed and characterized in two wild populations by using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Polymorphisms were evaluated in 24 individuals from two natural populations. Eleven of these primers generated polymorphic loci.
CONCLUSIONS
These microsatellite markers will be useful in future investigations into the population genetics and mating system of D. pavettifolia.
研究前提
为 Duperrea pavettifolia 开发微卫星引物将成为交配系统分析和保护研究的基础。
方法和结果
通过使用 Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) 协议，从两个野生种群中开发和表征了 19 个微卫星标记。在来自两个自然种群的 24 个个体中评估了多态性。其中 11 个引物产生了多态性位点。
结论
这些微卫星标记将在未来对 D. pavettifolia 的种群遗传学和交配系统的研究中很有用。
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