Can Erkan, Karacalar Ulviye, Saka Sahin, Firat Kursat
Faculty of Fisheries, Tunceli University, 62000 Tunceli, Turkey.
J Aquat Anim Health. 2012 Jun;24(2):129-33. doi: 10.1080/08997659.2012.675925.
The risk of fish pathogen transmission via eggs can be reduced by disinfection in ozonated seawater. The aim of this study was to determine the suitable conditions for ozone disinfection of the eggs of gilthead seabream Sparus aurata, sea bass Dicentrarchus labrax, red porgy Pagrus pagrus, and common dentex Dentex dentex. The eggs were disinfected with a concentration (C) of 0.5 mg of ozone/L of water at four different exposure times (T = 2, 4, 8, and 16 min). The hatching rate was determined in triplicate for each treatment. Bacterial colonies were counted on tryptic soy agar and thiosulfate-citrate-bile salts-sucrose agar. At the end of the experiment, bacterial load and hatching rate were assessed together to determine the optimal ozone treatment values, which were estimated in CT units (i.e., C [= 0.5 mg/L] x T [min]). Optimal values were CT 2-4 (T = 4-8 min; 18 degrees C) for gilthead seabream and red porgy, CT 2 (T = 4 min; 18 degrees C) for common dentex, and CT 4 (T = 8 min; 15 degrees C) for sea bass.
通过在臭氧处理过的海水中进行消毒，可以降低鱼类病原体通过鱼卵传播的风险。本研究的目的是确定对金头鲷、海鲈、红鲷和真鲷鱼卵进行臭氧消毒的合适条件。鱼卵在臭氧浓度（C）为0.5毫克/升水的情况下，分别暴露于四个不同的时间（T = 2、4、8和16分钟）进行消毒。每种处理重复测定三次孵化率。在胰蛋白胨大豆琼脂和硫代硫酸盐-柠檬酸盐-胆盐-蔗糖琼脂上计数细菌菌落。实验结束时，综合评估细菌载量和孵化率以确定最佳臭氧处理值，该值以CT单位估算（即C [= 0.5毫克/升] × T [分钟]）。金头鲷和红鲷的最佳值为CT 2 - 4（T = 4 - 8分钟；18摄氏度），真鲷的最佳值为CT 2（T = 4分钟；18摄氏度），海鲈的最佳值为CT 4（T = 8分钟；15摄氏度）。
