State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Anal Chim Acta. 2012 Sep 5;741:93-9. doi: 10.1016/j.aca.2012.06.048. Epub 2012 Jul 5.
In this article, we reported a novel approach for in situ labeling and imaging HeLa cancer cells utilizing a bifunctional aptamer (AS1411) and its fluorescent ligand, protoporphyrin IX (PPIX). In the presence of potassium ion, AS1411 folded to G-quadruplex structure, binded fluorescent ligand (PPIX) with fluorescent enhancement, and targeted the nucleolin overexpressed by cancer cells. Consequently, bioimaging of cancer cells specifically were realized by laser scanning confocal microscope. The bioimaging strategy with AS1411-PPIX complex was capable to distinguish HeLa cancer cells from normal cells unambiguously, and fluorescence imaging of cancer cells was also realized in human serum. Moreover, the bioimaging method was very facile, effective and need not any covalent modification. These results illustrated that the useful approach can provide a novel clue for bioimaging based on non-covalent bifunctional aptamer in clinic diagnosis.
在本文中，我们报道了一种利用双功能适体（AS1411）及其荧光配体原卟啉 IX（PPIX）原位标记和成像 HeLa 癌细胞的新方法。在钾离子存在下，AS1411 折叠成 G-四链体结构，与荧光配体（PPIX）结合产生荧光增强，并靶向癌细胞过度表达的核仁素。因此，通过激光扫描共聚焦显微镜实现了对癌细胞的特异性生物成像。AS1411-PPIX 复合物的生物成像策略能够明确地区分 HeLa 癌细胞和正常细胞，并且还可以在人血清中实现对癌细胞的荧光成像。此外，该生物成像方法非常简单、有效，且不需要任何共价修饰。这些结果表明，该方法为基于非共价双功能适体的临床诊断中的生物成像提供了新的线索。
