Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manangu, Anyangsi, Gyeonggido 430-757, Republic of Korea.
Vet Microbiol. 2012 Dec 28;161(1-2):222-8. doi: 10.1016/j.vetmic.2012.07.014. Epub 2012 Jul 17.
A total of 13 avian influenza viruses of the H10 subtype were isolated from wild birds in South Korea over the winter season between July 2008 and July 2011. The HA cleavage site of most of the isolated viruses, PEIMQGR↓G was similar to that of H10 viruses (A/turkey/England/384/79 and A/mandarin duck/Singapore/805/93), which are well known to be highly pathogenic in chickens. The exception was the A/mallard/Korea/1242/10(H10N6) virus, which had a PEMMQGR motif. Phylogenetic analysis showed that eight genes of the isolated H10 viruses belonged to the Eurasian lineage, and that the Korean H10 viruses could be divided four genotypes (genotypes A, B, C and D). Chicken challenge studies revealed that most of the H10 viruses did not replicate well through the natural infection route, but a genotype D virus was re-isolated from the brain of a chicken inoculated by the intravenous route. Although H10 viruses have not been isolated from poultry in South Korea, our results emphasize the continuing need to monitor the evolutionary genetics of the influenza virus in wild birds.
2008 年 7 月至 2011 年 7 月冬季期间，韩国从野鸟中总共分离到 13 株 H10 亚型禽流感病毒。大多数分离病毒的 HA 裂解位点，PEIMQGR↓G，类似于高致病性鸡流感病毒（A/火鸡/英格兰/384/79 和 A/麻鸭/新加坡/805/93）。例外的是 A/mallard/Korea/1242/10(H10N6)病毒，其具有 PEMMQGR 基序。系统进化分析表明，分离的 H10 病毒的 8 个基因属于欧亚谱系，韩国 H10 病毒可分为 4 种基因型（基因型 A、B、C 和 D）。鸡攻毒研究表明，大多数 H10 病毒通过自然感染途径不能很好地复制，但通过静脉途径接种的鸡脑重新分离到一株 D 基因型病毒。尽管韩国没有从家禽中分离到 H10 病毒，但我们的结果强调了继续监测野鸟中流感病毒进化遗传学的必要性。
