Joiner C H, Gunn R B, Fröhlich O
Department of Pediatrics, University of Cincinnati College of Medicine,Ohio 45267-0541.
Pediatr Res. 1990 Dec;28(6):587-90. doi: 10.1203/00006450-199012000-00008.
Anion transport in sickle cells (SS RBC) mediated by the band 3 membrane protein was evaluated by three different measures in both oxygenated and deoxygenated conditions and compared to normal red cells. First, Cl- self-exchange measured as 36Cl- efflux at 0 degrees C was normal in SS RBC in both Vmax and dependence on extracellular Cl- concentration. There was no effect of deoxygenation on either parameter. Second, stilbene-sensitive 35SO4=; SO4= exchange, measured at 37 degrees C where morphologic sickling occurred, was also unaffected by deoxygenation and was normal compared to normal red cells. Third, conductive Cl- flux was assessed by measuring the rates of Cl(-)-limited K+ efflux in valinomycin-treated cells at 37 degrees C. Both the stilbene-sensitive and insensitive components of net Cl- flux were similar in SS RBC and normal red cells, and were unaltered by morphologic sickling. Thus, despite dramatic alterations in cation transport in SS RBC and the demonstration of interaction between band 3 protein and sickle cell, anion transport functions appear to be normal in SS RBC and are unaffected by deoxygenation. These data suggest that the majority of the anion exchangers in SS RBC are functionally normal.
通过三种不同的方法在氧合和脱氧条件下评估了由带3膜蛋白介导的镰状细胞（SS红细胞）中的阴离子转运，并与正常红细胞进行了比较。首先，以0℃下的36Cl-外流测量的Cl-自交换在SS红细胞中的Vmax和对细胞外Cl-浓度的依赖性方面均正常。脱氧对这两个参数均无影响。其次，在37℃（发生形态学镰变的温度）下测量的对二苯乙烯敏感的35SO4=；SO4=交换也不受脱氧影响，与正常红细胞相比是正常的。第三，通过测量在37℃下缬氨霉素处理的细胞中Cl(-)限制的K+外流速率来评估传导性Cl-通量。SS红细胞和正常红细胞中净Cl-通量的对二苯乙烯敏感和不敏感成分均相似，并且不受形态学镰变的影响。因此，尽管SS红细胞中的阳离子转运发生了显著改变，并且证明了带3蛋白与镰状细胞之间存在相互作用，但SS红细胞中的阴离子转运功能似乎正常，并且不受脱氧影响。这些数据表明，SS红细胞中的大多数阴离子交换器功能正常。
