Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Biochem Biophys Res Commun. 2012 Aug 24;425(2):401-6. doi: 10.1016/j.bbrc.2012.07.110. Epub 2012 Jul 27.
In the present study, we investigated whether omentin affected the expression of intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in tumor necrosis factor-α (TNF-α) induced human umbilical vein endothelial cells (HUVECs). Our data showed that omentin decreased TNF-α-induced expression of ICAM-1 and VCAM-1 in HUVECs. In addition, omentin inhibited TNF-α-induced adhesion of THP-1 cells to HUVECs. Further, we found that omentin inhibited TNF-α-activated signal pathway of nuclear factor-κB (NF-κB) by preventing NF-κB inhibitory protein (IκBα) degradation and NF-κB/DNA binding activity. Omentin pretreatment significantly inhibited TNF-α-induced ERK activity and ERK phosphorylation in HUVECs. Pretreatment with PD98059 suppressed TNF-α-induced NF-κB activity. Omentin, NF-kB inhibitor (BAY11-7082) and ERK inhibitor (PD98059) reduced the up-regulation of ICAM-1 and VCAM-1 induced by TNF-α. These results suggest that omentin may inhibit TNF-α-induced expression of adhesion molecules in endothelial cells via blocking ERK/NF-κB pathway.
在本研究中，我们研究了网膜素是否影响肿瘤坏死因子-α（TNF-α）诱导的人脐静脉内皮细胞（HUVEC）中细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达。我们的数据表明，网膜素降低了 TNF-α诱导的 HUVEC 中 ICAM-1 和 VCAM-1 的表达。此外，网膜素抑制了 TNF-α诱导的 THP-1 细胞与 HUVEC 的黏附。此外，我们发现网膜素通过阻止 NF-κB 抑制蛋白（IκBα）降解和 NF-κB/DNA 结合活性来抑制 TNF-α激活的核因子-κB（NF-κB）信号通路。网膜素预处理显著抑制了 TNF-α诱导的 HUVEC 中 ERK 活性和 ERK 磷酸化。PD98059 的预处理抑制了 TNF-α诱导的 NF-κB 活性。网膜素、NF-κB 抑制剂（BAY11-7082）和 ERK 抑制剂（PD98059）降低了 TNF-α诱导的 ICAM-1 和 VCAM-1 的上调。这些结果表明，网膜素可能通过阻断 ERK/NF-κB 通路抑制 TNF-α诱导的内皮细胞黏附分子表达。
