Ma X, Chen S-Y, Bai S-Q, Zhang X-Q, Li D-X, Zhang C-B, Yan J-J
Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Ya'an, Sichuan, P.R. China.
Genet Mol Res. 2012 Aug 16;11(3):2708-18. doi: 10.4238/2012.June.27.5.
Genetic diversity of Elymus sibiricus (Poaceae) was examined in eight populations from the southeast Qinghai-Tibet Plateau. We detected 291 RAPD polymorphic loci in 93 samples. The percentage of polymorphic bands (PPB) was 79%. Genetic diversity (H(E)) was 0.264, effective number of alleles (N(E)) was 1.444, Shannon's information index (H(O)) was 0.398, and expected Bayesian heterozygosity (H(B)) was 0.371. At the population level, PPB = 51%, N(E) = 1.306, H(E) = 0.176, I = 0.263, and H(B) = 0.247. A high level of genetic differentiation was detected based on Nei's genetic diversity analysis (G(ST) = 32.0%), Shannon's index analysis (33.7%), and the Bayesian method (θ(B) = 33.5%). The partitioning of molecular variance by AMOVA demonstrated significant genetic differentiation within populations (60%) and among populations (40%). The average number of individuals exchanged between populations per generation (N(m)) was 1.06. The populations were found to share high levels of genetic identity. No significant correlation was found between geographic distance and pairwise genetic distance (r = 0.7539, P = 0.9996). Correlation analysis revealed a significant correlation (r = 0.762) between RAPD H(E) found in this study and ISSR H(E) values from a previous study.
对青藏高原东南部8个老芒麦（禾本科）种群的遗传多样性进行了研究。我们在93个样本中检测到291个RAPD多态性位点。多态性条带百分比（PPB）为79%。遗传多样性（H(E)）为0.264，有效等位基因数（N(E)）为1.444，香农信息指数（H(O)）为0.398，预期贝叶斯杂合度（H(B)）为0.371。在种群水平上，PPB = 51%，N(E) = 1.306，H(E) = 0.176，I = 0.263，H(B) = 0.247。基于Nei遗传多样性分析（G(ST) = 32.0%）、香农指数分析（33.7%）和贝叶斯方法（θ(B) = 33.5%）检测到高水平的遗传分化。通过AMOVA进行的分子方差分析表明，种群内（60%）和种群间（40%）存在显著的遗传分化。每代种群间交换的个体平均数量（N(m)）为1.06。发现这些种群具有较高的遗传同一性。地理距离与成对遗传距离之间未发现显著相关性（r = 0.7539，P = 0.9996）。相关性分析显示，本研究中发现的RAPD H(E)与先前研究中的ISSR H(E)值之间存在显著相关性（r = 0.762）。
