Commensal and Probiotics-Host Interactions Laboratory, INRA, UMR1319 Micalis, Jouy-en-Josas, France.
Appl Environ Microbiol. 2012 Oct;78(19):7148-51. doi: 10.1128/AEM.01076-12. Epub 2012 Jul 27.
Random insertional mutagenesis performed on a Lactococcus lactis reporter strain led us to identify L. lactis ybdD as a protein-overproducing mutant. In different expression contexts, the ybdD mutant shows increased levels of exported proteins and therefore constitutes a new and attractive heterologous protein production host. This study also highlights the importance of unknown regulatory processes that play a role during protein secretion.
随机插入突变在乳球菌报告菌株上进行，导致我们鉴定出乳球菌 ybdD 是一种蛋白质过表达突变体。在不同的表达环境中，ybdD 突变体显示出更高水平的分泌蛋白，因此构成了一种新的有吸引力的异源蛋白生产宿主。本研究还强调了在蛋白质分泌过程中起作用的未知调控过程的重要性。
