Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Anticancer Res. 2012 Aug;32(8):3097-101.
Protein kinase C (PKC)α is distributed in almost all tissues and participates in various signaling pathways. However, the role of PKCα in carcinogenesis remains unclear. In this study, we performed complete skin carcinogenesis in PKCα knockout mice by repeated administration of 7,12-dimethylbenz[a]anthracene (DMBA).
Complete skin carcinogenesis was performed by repeated DMBA treatment using PKCα knockout mice. The number of tumors was determined weekly. Tumor types were determined by Hematoxylin and eosin (H & E) analysis. Tumor growth was assayed by proliferating cell nuclear antigen (PCNA) staining.
In the knockout mice, the average number of tumors was 16.6/mouse at 20 weeks. In contrast, in the wild-type (WT) mice, the tumor number was 6.9/mouse. Growth and malignant grade of tumors in PKCα knockout mice did not differ from those in WT mice.
PKCα suppresses tumor formation, but not tumor growth and progression in skin carcinogenesis.
蛋白激酶 C（PKC）α几乎分布于所有组织中，并参与各种信号通路。然而，PKCα 在致癌作用中的角色尚不清楚。在这项研究中，我们通过重复给予 7,12-二甲基苯并蒽（DMBA），在 PKCα 敲除小鼠中进行了完整的皮肤致癌作用。
使用 PKCα 敲除小鼠通过重复 DMBA 处理进行完整的皮肤致癌作用。每周确定肿瘤数量。通过苏木精和伊红（H&E）分析确定肿瘤类型。通过增殖细胞核抗原（PCNA）染色测定肿瘤生长。
在敲除小鼠中，20 周时肿瘤的平均数量为 16.6/只。相比之下，在野生型（WT）小鼠中，肿瘤数量为 6.9/只。PKCα 敲除小鼠的肿瘤生长和恶性程度与 WT 小鼠没有差异。
PKCα 抑制肿瘤形成，但不影响皮肤致癌作用中的肿瘤生长和进展。
