Yiming Zhou, Lei Jiang, Renyou Zhai, Shan Ke, Xue Li, Min Liu, Jun Gao
Department of Radiology, Beijing Chao-Yang Hospital, Capital Medical University, Chaoyang District, Beijing 100020.
Oncol Lett. 2012 Aug;4(2):221-226. doi: 10.3892/ol.2012.729. Epub 2012 May 24.
The aim of this study was to investigate the changes in the uptake function of the liver in rats with obstructive jaundice prior to and following relief from obstructive jaundice, and to investigate whether superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance imaging (MRI) could be used to assess liver uptake function. In total, 40 male Sprague Dawley rats were randomly assigned into four groups: Obstructive jaundice, internal drainage, external drainage and sham surgery. The common bile ducts in the obstructive jaundice group were ligated. Internal drainage (ID) and external drainage (ED) groups were ligated, followed by internal drainage or external drainage, respectively. The T2 and T2* values of the liver parenchyma were measured. Liver sections were stained with Perls' Prussian blue, and hematoxylin and eosin. The number of SPIO-nanoparticle clusters was counted manually using a microscope. Total bilirubin of the blood was measured. Results showed that the T2 and T2* values and total bilirubin of the obstructive jaundice group were significantly higher compared to the other three groups. The number of SPIO-nanoparticle clusters in the obstructive jaundice group was significantly lower compared to the other three groups. In conclusion, obstructive jaundice suppresses liver uptake function in rats, which may be reversed by internal and external biliary drainage. However, no significant difference was found between the therapeutic effect of ID and ED on liver uptake function. Thus, SPIO-enhanced MRI may be used to evaluate the uptake function of the liver.
本研究的目的是调查阻塞性黄疸大鼠在阻塞性黄疸解除前后肝脏摄取功能的变化，并研究超顺磁性氧化铁（SPIO）增强磁共振成像（MRI）是否可用于评估肝脏摄取功能。总共40只雄性Sprague Dawley大鼠被随机分为四组：阻塞性黄疸组、内引流组、外引流组和假手术组。阻塞性黄疸组结扎胆总管。内引流（ID）组和外引流（ED）组先结扎胆总管，然后分别进行内引流或外引流。测量肝实质的T2和T2值。肝切片用Perls普鲁士蓝、苏木精和伊红染色。使用显微镜手动计数SPIO纳米颗粒簇的数量。测量血液中的总胆红素。结果显示，阻塞性黄疸组的T2和T2值以及总胆红素显著高于其他三组。阻塞性黄疸组的SPIO纳米颗粒簇数量显著低于其他三组。总之，阻塞性黄疸会抑制大鼠肝脏摄取功能，内、外胆道引流可能会使其逆转。然而，ID和ED对肝脏摄取功能的治疗效果之间未发现显著差异。因此，SPIO增强MRI可用于评估肝脏摄取功能。
