Szczubiał M, Dabrowski R, Kankofer M, Bochniarz M, Komar M
Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Głeboka 30, 20-612 Lublin, Poland.
Pol J Vet Sci. 2012;15(2):291-6. doi: 10.2478/v10181-011-0149-x.
The aim of the present study was to determine the concentration of serum amyloid A (SAA) and the activity of ceruloplasmin (Cp) in milk from cows with subclinical mastitis caused by different pathogens. Eighty-four milk samples from cows with subclinical mastitis and fourteen milk samples from healthy cows were examined. SAA concentration was determined using the commercial ELISA kit (Tridelta Development Ltd., Greystones, Wicklow, Ireland). Cp activity was assessed spectrophotometrically, using the Rice method. The results reveal that the concentration of SAA (with exception of CNS) and activity of Cp in cow milk can be regarded as markers of subclinical mastitis, irrespective of the microorganism inducing the disease. In conclusion, measurement of SAA and Cp in milk samples could be a useful method in diagnosing subclinical mastitis in cows, but the method should be adapted for field use.
本研究的目的是测定由不同病原体引起的亚临床型乳腺炎奶牛乳汁中血清淀粉样蛋白A（SAA）的浓度和铜蓝蛋白（Cp）的活性。对84份来自亚临床型乳腺炎奶牛的乳汁样本和14份来自健康奶牛的乳汁样本进行了检测。使用商用ELISA试剂盒（Tridelta Development Ltd.，爱尔兰威克洛郡格雷斯顿斯）测定SAA浓度。采用赖斯法通过分光光度法评估Cp活性。结果表明，无论引发疾病的微生物是什么，奶牛乳汁中SAA的浓度（中枢神经系统感染除外）和Cp的活性都可被视为亚临床型乳腺炎的标志物。总之，检测乳汁样本中的SAA和Cp可能是诊断奶牛亚临床型乳腺炎的一种有用方法，但该方法应进行调整以适用于现场使用。
