Roussan D A, Shaheen I A, Khawaldeh G Y, Totanji W S, Al-Rifai R H
Technical Department, Provimi, Jordan, P.O. Box 499, Amman 11118, Jordan.
Pol J Vet Sci. 2012;15(2):337-44. doi: 10.2478/v10181-012-0052-0.
Enteric diseases cause substantial economic losses to the poultry industry. Astroviruses, rotaviruses, reoviruses, and adenovirus type 1 have been reported as a significant cause of intestinal symptoms in poultry. In the present study, intestinal samples from 70 commercial broiler chicken flocks were examined for the presence of astroviruses, rotavirus, and reovirus by reverse transcription-polymerase chain reaction, and for the presence of group I adenovirus by polymerase chain reaction. Astroviruses were identified in 38.6% of samples tested. Both avian nephritis virus and chicken astrovirus were identified in the astrovirus positive flocks, where 74.1% of these flocks were positive for only one type of astrovirus, whereas, 25.9% of these flocks were positive for both types of astrovirus. Reoviruses, rotaviruses, and adenoviruses were identified in 21.4, 18.6, and 14.3% of these flocks, respectively. Concomitant infection with two or more viruses in the same flock were also prominent, where 5.7, 5.7, 2.9, 2.9, 1.4, and 1.4% of these flocks were positive with both astrovirus and rotavirus; astrovirus and adenovirus; astrovirus and reovirus; rotavirus and adenovirus; rotavirus and reovirus; and reovirus and adenovirus respectively. Moreover, 4.3 and 2.7% of these flocks were positive for astrovirus, reovirus, and adenovirus; and astrovirus, reovirus, and rotavirus, respectively. Further studies will focus on identifying specific viral factors or subtypes/subgroups associated with disease through pathogenesis studies, economic losses caused by infections and co-infections of these pathogens, and the costs and benefits of countermeasures.
肠道疾病给家禽业造成了巨大的经济损失。星状病毒、轮状病毒、呼肠孤病毒和1型腺病毒已被报道为家禽肠道症状的重要病因。在本研究中，通过逆转录聚合酶链反应检测了70个商品肉鸡群的肠道样本中星状病毒、轮状病毒和呼肠孤病毒的存在情况，并通过聚合酶链反应检测了I群腺病毒的存在情况。在38.6%的检测样本中发现了星状病毒。在星状病毒阳性鸡群中同时鉴定出禽肾炎病毒和鸡星状病毒，其中74.1%的鸡群仅对一种星状病毒呈阳性，而25.9%的鸡群对两种星状病毒均呈阳性。呼肠孤病毒、轮状病毒和腺病毒分别在21.4%、18.6%和14.3%的鸡群中被鉴定出。同一鸡群中两种或更多种病毒的混合感染也很突出，其中5.7%、5.7%、2.9%、2.9%、1.4%和1.4%的鸡群分别对星状病毒和轮状病毒；星状病毒和腺病毒；星状病毒和呼肠孤病毒；轮状病毒和腺病毒；轮状病毒和呼肠孤病毒；呼肠孤病毒和腺病毒呈阳性。此外，4.3%和2.7%的鸡群分别对星状病毒、呼肠孤病毒和腺病毒；星状病毒、呼肠孤病毒和轮状病毒呈阳性。进一步的研究将集中于通过发病机制研究确定与疾病相关的特定病毒因子或亚型/亚组，这些病原体的感染和混合感染所造成的经济损失，以及应对措施的成本和效益。
