Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, Eumsung, Chungbuk, Republic of Korea.
Foodborne Pathog Dis. 2012 Aug;9(8):686-91. doi: 10.1089/fpd.2011.1119. Epub 2012 Jul 30.
Tetrandrine (TET) is a bis-benzylisoquinoline alkaloid derived from the radix of Stephania tetrandra S. Moore. TET performs a wide spectrum of biological activities. The radix of S. tetrandrae has been used traditionally in Asia, including Korea, to treat congestive circulatory disorders and inflammatory diseases. The aim of this study was to examine the mechanism of antibacterial activity of tetrandrine against Staphylococcus aureus. The mechanism was investigated by studying the effects of TET in combination with detergent or membrane potential un-couplers. In addition, the direct involvement of peptidoglycan (PGN) was assessed in titration assays. TET activity against S. aureus was 125-250 μg/mL, and the minimum inhibitory concentration (MIC) of the two reference strains was 250 μg/mL. The OD(600) of each suspension treated with a combination of ethylenediaminetetraacetic acid (EDTA), tris(hydroxymethyl) aminomethane (TRIS), and Triton X-100 (TX) with TET (0.25×MIC) had been reduced from 43% to 96%. Additional structure-function studies on the antibacterial activity of TET in combination with other agents may lead to the discovery of more effective antibacterial agents.
汉防己甲素（TET）是一种双苄基异喹啉生物碱，来源于粉防己 Stephania tetrandra S. Moore 的根。TET 具有广泛的生物活性。在亚洲，包括韩国在内，粉防己的根传统上被用于治疗充血性循环障碍和炎症性疾病。本研究旨在研究汉防己甲素对金黄色葡萄球菌的抗菌活性的作用机制。通过研究 TET 与去污剂或膜电位解偶联剂联合使用的效果来研究该机制。此外，在滴定测定中评估了肽聚糖（PGN）的直接参与。TET 对金黄色葡萄球菌的活性为 125-250μg/mL，两种参考菌株的最小抑菌浓度（MIC）为 250μg/mL。用乙二胺四乙酸（EDTA）、三羟甲基氨基甲烷（TRIS）和 Triton X-100（TX）与 TET（0.25×MIC）组合处理的每个悬浮液的 OD（600）从 43%降低到 96%。对 TET 与其他药物联合使用的抗菌活性进行更多的结构功能研究，可能会发现更有效的抗菌药物。
