Henry Wellcome Laboratories for Integrative Neuroscience & Endocrinology & MRC Centre for Synaptic Plasticity, Dorothy Hodgkin Building, University of Bristol, Whitson Street, Bristol BS1 3NY, UK.
Eur J Neurosci. 2012 Oct;36(7):2941-8. doi: 10.1111/j.1460-9568.2012.08220.x. Epub 2012 Jul 30.
Evidence suggests that the acquisition of recognition memory depends upon CREB-dependent long-lasting changes in synaptic plasticity in the perirhinal cortex.The CREB-responsive microRNA miR-132 has been shown to regulate synaptic transmission and we set out to investigate a role for this microRNA in recognition memory and its underlying plasticity mechanisms. To this end we mediated the specific overexpression of miR-132 selectively in the rat perirhinal cortex and demonstrated impairment in short-term recognition memory. This functional deficit was associated with a reduction in both long-term depression and long-term potentiation. These results confirm that microRNAs are key coordinators of the intracellular pathways that mediate experience-dependent changes in the brain. In addition, these results demonstrate a role for miR-132 in the neuronal mechanisms underlying the formation of short-term recognition memory.
有证据表明，识别记忆的获得依赖于海人酸杏仁核外侧区突触可塑性的 CREB 依赖性长期变化。已经表明 CREB 反应性 microRNA miR-132 调节突触传递，我们着手研究这种 microRNA 在识别记忆及其潜在可塑性机制中的作用。为此，我们选择性地在大鼠海人酸杏仁核外侧区中介导 miR-132 的特异性过表达，证明短期识别记忆受损。这种功能缺陷与长时程压抑和长时程增强的减少有关。这些结果证实了 microRNAs 是介导大脑中依赖经验的变化的细胞内途径的关键协调者。此外，这些结果表明 miR-132 在形成短期识别记忆的神经元机制中起作用。
