Laboratory of Clinical and Experimental Biochemistry, Reference Center for Lactobacilli, Tucuman, Argentina.
Microbiol Immunol. 2012 Nov;56(11):771-81. doi: 10.1111/j.1348-0421.2012.00495.x.
Lactobacillus rhamnosus CRL1505 (Lr1505), L. rhamnosus CRL1506 (Lr1506) and L. casei CRL431 (Lc431) are able to stimulate intestinal immunity, but only Lr1505 and Lc431 are able to stimulate immunity in the respiratory tract. With the aim of advancing the understanding of the immunological mechanisms involved in stimulation of distant mucosal sites, this study evaluated the effects of orally administered probiotics on the functions of alveolar and peritoneal macrophages. Compared to a control group, these three lactobacilli were able to significantly increase phagocytic and microbicidal activities of peritoneal macrophages. After intraperitoneal challenge with pathogenic Candida albicans, mice treated with immunobiotics had significantly lower pathogen counts in infected organs. Moreover, lactobacilli-treated mice had a stronger immune response against C. albicans. On the other hand, only Lc1505 and Lc431 were able to improve activity of and cytokine production by alveolar macrophages. Only in these two groups was there better resistance to respiratory challenge with C. albicans, which correlated with improved respiratory immune response. The results of this study suggest that consumption of some probiotic strains could be useful for improving resistance to infections in sites distant from the gut by increasing the activity of macrophages at those sites.
鼠李糖乳杆菌 CRL1505（Lr1505）、鼠李糖乳杆菌 CRL1506（Lr1506）和干酪乳杆菌 CRL431（Lc431）能够刺激肠道免疫，但只有 Lr1505 和 Lc431 能够刺激呼吸道免疫。为了深入了解刺激远隔黏膜部位所涉及的免疫机制，本研究评估了口服益生菌对肺泡和腹腔巨噬细胞功能的影响。与对照组相比，这三种乳杆菌能够显著提高腹腔巨噬细胞的吞噬和杀菌活性。经腹腔内接种致病性白色念珠菌后，用免疫生物制剂治疗的小鼠感染器官中的病原体数量明显降低。此外，用乳杆菌处理的小鼠对白色念珠菌产生了更强的免疫反应。另一方面，只有 Lc1505 和 Lc431 能够提高肺泡巨噬细胞的活性和细胞因子的产生。只有在这两组中，对白色念珠菌的呼吸道挑战的抵抗力更好，这与呼吸免疫反应的改善相关。本研究结果表明，通过增加这些部位巨噬细胞的活性，某些益生菌株的消耗可能有助于提高远隔于肠道的感染部位的抵抗力。
