SOX17甲基化抑制其在肺癌中对Wnt信号通路的拮抗作用。
SOX17 methylation inhibits its antagonism of Wnt signaling pathway in lung cancer.
作者信息
Yin Dongtao, Jia Yan, Yu Yuanzi, Brock Malcolm V, Herman James G, Han Chao, Su Xiaomo, Liu Yang, Guo Mingzhou
机构信息
Department of Thoracic Surgery, Chinese PLA General Hospital, Beijing 100853, China.
出版信息
Discov Med. 2012 Jul;14(74):33-40.
Abstract
The purpose of this study was to explore epigenetic changes and functions of SOX17 in human lung cancer. Five lung cancer cell lines and 88 primary lung cancer samples were examined in this study. Methylation-specific polymerase chain reaction (MSP), semi-quantitative reverse-transcription PCR, immunohistochemistry, luciferase reporter assays, colony-formation assays, and western blotting were used to analyze methylation changes and functions of SOX17 in lung cancer. SOX17 methylation was found in 60.2% of primary human lung cancer samples, and promoter region methylation of SOX17 silenced its expression. SOX17 methylation was associated with female patients and lung cancer differentiation. Colony-formation assays revealed that SOX17 suppressed lung cancer cell proliferation. Re-expression of SOX17 inhibited Wnt signaling in H23 lung cancer cell line. SOX17 acts as a Wnt signaling inhibitor.
摘要
本研究的目的是探讨SOX17在人类肺癌中的表观遗传变化及其功能。本研究检测了五种肺癌细胞系和88例原发性肺癌样本。采用甲基化特异性聚合酶链反应（MSP）、半定量逆转录PCR、免疫组织化学、荧光素酶报告基因检测、集落形成检测和蛋白质免疫印迹法分析SOX17在肺癌中的甲基化变化及其功能。在60.2%的原发性人类肺癌样本中发现了SOX17甲基化，且SOX17的启动子区域甲基化使其表达沉默。SOX17甲基化与女性患者及肺癌分化相关。集落形成检测显示，SOX17抑制肺癌细胞增殖。SOX17的重新表达抑制了H23肺癌细胞系中的Wnt信号传导。SOX17作为一种Wnt信号抑制剂发挥作用。
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