Department of Obstetrics and Gynecology, American University of Beirut Medical Center, Beirut, Lebanon.
Neurourol Urodyn. 2013 Mar;32(3):287-92. doi: 10.1002/nau.22288. Epub 2012 Jul 27.
To examine the effect of epigallocatechin gallate (EGCG), a green tea catechin, on the bladder of rats exposed to water avoidance stress (WAS).
Twenty female Sprague-Dawley rats were divided into four groups of five. The first group was exposed to WAS for7 days. The second group was pretreated with EGCG 1 mg/kg intraperitoneally (IP) for 7 days before exposure to WAS. The treatment was continued till the end of the experiment. The third group was placed on the platform in a container without water for 2 hr daily for 7 days (Sham WAS). The fourth group was pretreated with saline I.P. for 7 days before being exposed to sham WAS.
Bladder wall evaluation for signs of inflammation and total and activated mast cell counts. Secondary outcome: fecal pellet output and micturition frequency at baseline, day 1 and day 7.
Bladder walls from rats exposed to WAS revealed significantly higher inflammation score, total and degranulated mast cell counts compared to the sham WAS group. EGCG administration had an obvious protective effect on the bladder mucosa, as the inflammation score, total and degranulated mast cell counts were all significantly lower than in the WAS group. In the WAS group, fecal pellet output and micturition frequency increased above baseline throughout the experiment. Comparison of sham WAS group versus sham WAS with saline revealed no statistically significant difference in any parameter.
EGCG given at 1 mg/kg I.P to rats has a significant protective effect against bladder degenerative changes following WAS.
研究表没食子儿茶素没食子酸酯（EGCG），一种绿茶儿茶素，对暴露于水回避应激（WAS）的大鼠膀胱的影响。
将 20 只雌性 Sprague-Dawley 大鼠分为五组，每组 5 只。第一组暴露于 WAS 7 天。第二组在暴露于 WAS 前，腹腔内（IP）给予 EGCG 1mg/kg，预处理 7 天。治疗一直持续到实验结束。第三组每天在容器中无水上的平台上放置 2 小时（假 WAS）。第四组在暴露于假 WAS 前，IP 给予生理盐水预处理 7 天。
评估膀胱壁炎症迹象和总及活化肥大细胞计数。次要结果：基础时、第 1 天和第 7 天的粪便颗粒输出和排尿频率。
与假 WAS 组相比，暴露于 WAS 的大鼠的膀胱壁显示出明显更高的炎症评分、总及脱颗粒肥大细胞计数。EGCG 给药对膀胱黏膜具有明显的保护作用，因为炎症评分、总及脱颗粒肥大细胞计数均明显低于 WAS 组。在 WAS 组中，粪便颗粒输出和排尿频率在整个实验过程中均高于基础值。与假 WAS 组相比，假 WAS 与生理盐水组之间的比较，任何参数均无统计学差异。
在 1mg/kg IP 给予大鼠 EGCG 对 WAS 后膀胱退行性变化具有显著的保护作用。
