从乳球菌（Lactococcus lactis）中生产和分离二肽基肽酶 IV：用于工业生产的放大。

Production and separation of dipeptidyl peptidase IV from Lactococcus lactis: scale up for industrial production.

机构信息

Department of Food Engineering, Engineering Faculty, Pamukkale University, Kinikli, Denizli, Turkey.

出版信息

Bioprocess Biosyst Eng. 2012 Oct;35(8):1417-27. doi: 10.1007/s00449-012-0730-4. Epub 2012 Jul 31.

Abstract

Lactococcus lactis spp. lactis and Lactococcus lactis spp. cremoris are widely used in the manufacture of fermented milk. These strains were compared for production of Dipeptidyl Peptidase IV (DPP IV) enzyme in terms of enzyme activity, specific growth rates and productivity. Lactococcus lactis spp. lactis was produced in 3 L bioreactor and scaled up to 30 and 150 L stirred tank bioreactors, and the enzyme activities were found as 110, 110 and 122 mU mL(-1), respectively. After 8 h of production, separation steps were performed. While purification fold was 127 and yield was 2.69 %, the molecular weight of the enzyme was estimated as 68 kDa. Partially purified enzyme was enteric coated with capsules and a 95.5 % of DPP IV enzyme passed into the artificial intestine. Results show that production of DPP IV enzyme by Lactococcus lactis spp. lactis strain in submerged culture is comparable with the productions by commercial strains, mostly Aspergillus, in solid state fermentations based on productivity.

摘要

乳球菌（Lactococcus lactis）亚种。乳脂链球菌和乳球菌（Lactococcus lactis）亚种。cremoris 广泛用于发酵乳的生产。在酶活性、比生长速率和生产力方面，比较了这些菌株生产二肽基肽酶 IV（DPP IV）酶的情况。乳球菌（Lactococcus lactis）亚种。在 3 L 生物反应器中生产乳脂链球菌，并放大到 30 和 150 L 搅拌罐生物反应器，酶活性分别为 110、110 和 122 mU mL(-1)。生产 8 小时后，进行分离步骤。虽然纯化倍数为 127，产率为 2.69%，但酶的分子量估计为 68 kDa。部分纯化的酶用胶囊肠溶性包衣，95.5%的 DPP IV 酶进入人工肠道。结果表明，在浸没培养中，乳球菌（Lactococcus lactis）亚种。乳脂链球菌菌株生产 DPP IV 酶的产率与基于生产力的固态发酵中商业菌株（主要是 Aspergillus）的产率相当。